免疫过氧化物酶染色法检测犬类自身免疫性皮肤病中的自身抗体；与免疫荧光结果的比较。

Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; comparison to immunofluorescence results.

作者信息

Bradley G A, Mays M B

机构信息

Department of Comparative and Experimental Pathology, College of Veterinary Medicine, University of Florida, Gainesville 32610.

出版信息

Vet Immunol Immunopathol. 1990 Oct;26(2):105-13. doi: 10.1016/0165-2427(90)90060-6.

DOI:10.1016/0165-2427(90)90060-6

PMID:2260277

Abstract

Skin sections from 22 dogs with autoimmune skin disease were stained with anti-canine IgG, IgM and IgA using an immunobridge immunoperoxidase method. Eight cases of lupus erythematosus, three cases of pemphigus vulgaris, and 11 cases of pemphigus foliaceus were included. Results of previously performed, direct immunofluorescence tests for the detection of canine immunoglobulin on skin were available on 17/22 cases. The immunoperoxidase method yielded an overall positive result in 59% (5/8 lupus erythematosus, 2/3 pemphigus vulgaris and 6/11 pemphigus foliaceus) versus an overall positive result of 47% for direct immunofluorescence (3/5 lupus erythematosus, 2/2 pemphigus vulgaris and 2/10 pemphigus foliaceus). The immunobridge immunoperoxidase method compared favorably to direct immunofluorescence testing of canine skin for autoantibody in cases of lupus erythematosis and pemphigus vulgaris, and was superior in cases of pemphigus foliaceus. This method should prove useful as an aid in the diagnosis of canine autoimmune skin disease.

摘要

采用免疫桥免疫过氧化物酶法，用抗犬IgG、IgM和IgA对22只患有自身免疫性皮肤病的犬的皮肤切片进行染色。其中包括8例红斑狼疮、3例寻常型天疱疮和11例落叶型天疱疮。22例中有17例可获得先前进行的用于检测皮肤中犬免疫球蛋白的直接免疫荧光试验结果。免疫过氧化物酶法的总体阳性率为59%（8例红斑狼疮中有5例、3例寻常型天疱疮中有2例、11例落叶型天疱疮中有6例），而直接免疫荧光法的总体阳性率为47%（5例红斑狼疮中有3例、2例寻常型天疱疮中有2例、10例落叶型天疱疮中有2例）。在红斑狼疮和寻常型天疱疮病例中，免疫桥免疫过氧化物酶法与犬皮肤自身抗体的直接免疫荧光检测相比效果良好，在落叶型天疱疮病例中则更具优势。该方法应被证明有助于犬自身免疫性皮肤病的诊断。