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通过固定化全细胞生物催化剂对d，l-泛内酯进行工业动力学拆分

Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst.

作者信息

Zhang Qiu-Hua, Yang Liu, Tang Yi-Bin, Huang Liu-Nv, Luo Wen-Fang

机构信息

Brother Research Center, Jiangxi Brother Pharmaceutical Co., Ltd Jiujiang 332700 China

出版信息

RSC Adv. 2021 Sep 13;11(48):30373-30376. doi: 10.1039/d1ra05708a. eCollection 2021 Sep 6.

DOI:10.1039/d1ra05708a
PMID:35480294
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9041136/
Abstract

Immobilized whole-cells of harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity.

摘要

携带重组d-内酯酶的固定化全细胞被包埋在海藻酸钙凝胶中，并用作催化动力学拆分d,l-泛内酯的高效生物催化剂。该固定化全细胞生物催化剂表现出良好的催化稳定性，用于d-泛内酯的立体选择性水解，可进行多达56次重复分批反应，而催化活性和对映选择性没有明显损失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/80d775cb8a24/d1ra05708a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/653affa82e79/d1ra05708a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/3374e9d960ba/d1ra05708a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/4eb016b304bb/d1ra05708a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/64bc1789c088/d1ra05708a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/80d775cb8a24/d1ra05708a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/653affa82e79/d1ra05708a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/3374e9d960ba/d1ra05708a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/4eb016b304bb/d1ra05708a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/64bc1789c088/d1ra05708a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5236/9041136/80d775cb8a24/d1ra05708a-f4.jpg

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本文引用的文献

1
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RSC Adv. 2020 Dec 24;11(2):721-725. doi: 10.1039/d0ra09053k.
2
Directed evolution and characterization of a novel D-pantonohydrolase from Fusarium moniliforme.来自串珠镰刀菌的新型D-泛解酸水解酶的定向进化与表征
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Practical resolution system for DL-pantoyl lactone using the lactonase from Fusarium oxysporum.利用尖孢镰刀菌内酯酶的DL-泛酰内酯实用拆分系统。
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Lactone-ring-cleaving enzyme: genetic analysis, novel RNA editing, and evolutionary implications.内酯环裂解酶：遗传分析、新型RNA编辑及进化意义
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