长链非编码 RNA MALAT1 通过海绵吸附 miR-129-5p 来介导骨间充质干细胞的成骨分化。

lncRNA MALAT1 mediates osteogenic differentiation of bone mesenchymal stem cells by sponging miR-129-5p.

机构信息

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai, China.

Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai, China.

出版信息

PeerJ. 2022 Apr 22;10:e13355. doi: 10.7717/peerj.13355. eCollection 2022.

BACKGROUND

Bone mesenchymal stem cells (BMSCs) have good osteogenic differentiation potential and have become ideal seed cells in bone tissue engineering. However, the osteogenic differentiation ability of BMSCs gradually weakens with age, and the regulatory mechanism is unclear.

METHOD

We conducted a bioinformatics analysis, dual-luciferase reporter (DLR) experiment, and RNA binding protein immunoprecipitation (RIP) to explore the hub genes that may affect BMSC functions.

RESULTS

The expression level of long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 () was significantly higher in the BMSCs from elderly than younger mice, while showed the opposite trend. The results of alkaline phosphatase staining, quantitative reverse transcription PCR and western blot experiments indicated that inhibiting the expression of inhibits the osteogenic differentiation of BMSCs. This effect can be reversed by reducing the expression of . Additionally, DLR and RIP experiments confirmed that acts as a sponge for .

CONCLUSION

Overall, our study findings indicated that lncRNA may play a critical role in maintaining the osteoblast differentiation potential of BMSCs by sponging .

背景

骨髓间充质干细胞（BMSCs）具有良好的成骨分化潜能，已成为骨组织工程中理想的种子细胞。然而，BMSCs 的成骨分化能力随年龄增长而逐渐减弱，其调控机制尚不清楚。

方法

我们进行了生物信息学分析、双荧光素酶报告（DLR）实验和 RNA 结合蛋白免疫沉淀（RIP）实验，以探讨可能影响 BMSC 功能的关键基因。

结果

长链非编码 RNA（lncRNA）转移相关肺腺癌转录本 1（）在老年小鼠的 BMSCs 中的表达水平明显高于年轻小鼠，而则呈现相反的趋势。碱性磷酸酶染色、定量逆转录 PCR 和 Western blot 实验结果表明，抑制的表达可抑制 BMSCs 的成骨分化。这种作用可以通过降低的表达来逆转。此外，DLR 和 RIP 实验证实，作为的海绵体起作用。