Huang Ying, Zou Zhaoyong, Ping Hang, Lei Liwen, Xie Jingjing, Xie Hao, Fu Zhengyi
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology Wuhan 430070 China
School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology Wuhan 430070 China
RSC Adv. 2021 May 24;11(30):18590-18596. doi: 10.1039/d1ra02199k. eCollection 2021 May 19.
Silk fibroin is a promising biomaterial that has been used for tissue engineering applications. However, the influence of silk fibroin on the mineralization of calcium phosphate in different biological environments has not been discussed before. In this work, we fabricated organized silk fibroin film as the organic framework and amorphous calcium phosphate (ACP) deposited on the films as precursors. The transformation pathways and morphology of ACP was then studied in both enzyme and PBS (phosphate buffer saline) solutions. While only hydroxyapatite (HA) crystals formed in enzyme solution, a mixture of tricalcium phosphate (TCP) and HA crystals were obtained in PBS solution, which can be related to the variations of the content of silk fibroin and pH of the solution. Therefore, silk fibroin films can have an important effect on the mineralization process of calcium phosphate in different biological environments. In addition, cell cultivation experiments show that the silk films after mineralization promoted osteogenesis and exhibited good biocompatibility.
丝素蛋白是一种很有前景的生物材料，已被用于组织工程应用。然而，丝素蛋白在不同生物环境中对磷酸钙矿化的影响此前尚未被讨论过。在这项工作中，我们制备了有序的丝素蛋白膜作为有机框架，并将无定形磷酸钙（ACP）沉积在膜上作为前体。然后在酶溶液和磷酸盐缓冲盐水（PBS）溶液中研究了ACP的转变途径和形态。在酶溶液中仅形成了羟基磷灰石（HA）晶体，而在PBS溶液中获得了磷酸三钙（TCP）和HA晶体的混合物，这可能与丝素蛋白含量和溶液pH值的变化有关。因此，丝素蛋白膜在不同生物环境中对磷酸钙的矿化过程可能具有重要影响。此外，细胞培养实验表明，矿化后的丝素膜促进了骨生成并表现出良好的生物相容性。
