用合成两性离子对不稳定的N-乙酰葡糖胺基转移酶-V进行低温储存。
Cryostorage of unstable -acetylglucosaminyltransferase-V by synthetic zwitterions.
作者信息
Hirata Tetsuya, Takekiyo Takahiro, Yoshimura Yukihiro, Tokoro Yuko, Ishizaki Takeru, Kizuka Yasuhiko, Kuroda Kosuke
机构信息
Institute for Glyco-core Research (iGCORE), Gifu University Gifu Gifu 501-1193 Japan
Department of Applied Chemistry, National Defense Academy Yokosuka Kanagawa 239-8686 Japan
出版信息
RSC Adv. 2022 Apr 14;12(19):11628-11631. doi: 10.1039/d2ra01575g. eCollection 2022 Apr 13.
We report biocompatible materials for cryostorage of unstable proteins such as cancer-related enzyme, -acetylglucosaminyltransferase-V (GnT-V). GnT-V activity and the amount of protein after freezing were better retained in synthetic zwitterion solutions than in the glycerol solution. This study highlights the potential utility of synthetic zwitterions as novel cryoprotectants.
我们报道了用于不稳定蛋白质（如癌症相关酶β-乙酰氨基葡萄糖转移酶-V（GnT-V））低温储存的生物相容性材料。与甘油溶液相比，合成两性离子溶液在冷冻后能更好地保留GnT-V活性和蛋白质含量。这项研究突出了合成两性离子作为新型冷冻保护剂的潜在用途。
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