Center of Medical Sciences and Pharmaceutical, Western Paraná State University, Rua Universitária, 2069, Cascavel, PR, ZC 85814-110, Brazil.
Biotechnology Bioprocess and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande Do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, ZC 91501-970, Brazil.
Braz J Microbiol. 2022 Sep;53(3):1167-1174. doi: 10.1007/s42770-022-00754-w. Epub 2022 Apr 28.
Purified endoxylanase from Thermomyces lanuginosus PC7S1T was immobilized in calcium alginate, resulting in a yield of 78.5% and a reusability for 11 cycles. The stability of the immobilized enzyme was given for a pH range of 4 to 9 for 96 h. Endoxylanase immobilized in calcium alginate at 65 °C exhibited thermal stability equal to the soluble enzyme for 5 h, and at high temperatures of 75 °C and 85 °C showed half-lives of 4 and 3 h, respectively. Both soluble endoxylanase and immobilized forms were able to hydrolyze hemicellulose, obtained from low-lignin sorghum biomass pretreated with 5% HO and 2% NaOH, after 1 h of incubation at 65 °C, releasing a mixture of short-chain xylooligosaccharides (X2-X6). The highest amounts of XOS generated were those for X5 (24 to 40%), X4 (33 to 39%), and X3 (11 to 22%). These XOS acted as prebiotics, promoting the growth of the probiotic L. acidophilus, similar to glucose in the MRS broth. These results show the potential of low-lignin sorghum to generate XOS with prebiotic activity, suggesting the application of these compounds in the food industry.
从嗜热丝孢菌 PC7S1T 中纯化的内切木聚糖酶被固定在海藻酸钙中，产率为 78.5%，可重复使用 11 次。固定化酶的稳定性在 pH4 到 9 范围内保持 96 小时。固定在海藻酸钙中的内切木聚糖酶在 65°C 下表现出与可溶性酶相同的热稳定性，5 小时后，在 75°C 和 85°C 的高温下，半衰期分别为 4 小时和 3 小时。可溶性内切木聚糖酶和固定化形式都能够水解半纤维素，半纤维素是从用 5%HO 和 2%NaOH 预处理的低木质素高粱生物质中获得的，在 65°C 孵育 1 小时后，释放出短链木二糖寡糖（X2-X6）的混合物。生成的 XOS 中 X5（24%至 40%）、X4（33%至 39%）和 X3（11%至 22%）的含量最高。这些 XOS 作为益生元，促进了益生菌嗜酸乳杆菌的生长，类似于 MRS 肉汤中的葡萄糖。这些结果表明低木质素高粱具有产生具有益生元活性的 XOS 的潜力，这表明这些化合物在食品工业中的应用。
