人脐带间充质干细胞来源的微小 RNA-140-5p 释放的外泌体抑制子痫前期的发展。
microRNA-140-5p from human umbilical cord mesenchymal stem cells-released exosomes suppresses preeclampsia development.
机构信息
Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, No.238 Jiefang Road, Wuchang, Wuhan, 430000, Hubei, China.
Department of Urology, General Hospital of Central Command Theater in PLA, Wuhan, 430000, Hubei, China.
出版信息
Funct Integr Genomics. 2022 Oct;22(5):813-824. doi: 10.1007/s10142-022-00848-6. Epub 2022 Apr 28.
This work unraveled the action of human umbilical cord mesenchymal stem cells-released exosomes (huc-MSCs-EXO) transfer of miR-140-5p in preeclampsia (PE). miR-140-5p and follistatin-like 3 (FSTL3) expression in placental tissues of PE patients was tested. EXO were isolated from huc-MSCs. Hypoxic trophoblast cells were co-cultured with huc-MSCs-EXO. Cell biological functions, angiogenesis, and inflammation were evaluated. Suppressed miR-140-5p and induced FSTL3 levels were measured in PE. Huc-MSCs-EXO drove biological functions and angiogenesis while hindering inflammation in hypoxic trophoblast cells. Increasing miR-140-5p further improved the positive role of huc-MSCs-EXO for hypoxic trophoblast cells, but the miR-140-5p-mediated effect in hypoxic trophoblast cells was abrogated by overexpressing FSTL3. miR-140-5p from huc-MSCs-EXO suppresses PE through repressing FSTL3.
本研究揭示了人脐带间充质干细胞来源的细胞外囊泡（huc-MSCs-EXO）中转录 miR-140-5p 在子痫前期（PE）中的作用。检测了 PE 患者胎盘组织中 miR-140-5p 和卵泡抑素样蛋白 3（FSTL3）的表达。从 huc-MSCs 中分离出 EXO。将缺氧滋养细胞与 huc-MSCs-EXO 共培养。评估细胞生物学功能、血管生成和炎症。PE 中检测到 miR-140-5p 受抑制和 FSTL3 水平升高。huc-MSCs-EXO 促进缺氧滋养细胞的生物学功能和血管生成，同时抑制炎症。增加 miR-140-5p 进一步增强了 huc-MSCs-EXO 对缺氧滋养细胞的积极作用，但过表达 FSTL3 则削弱了 miR-140-5p 对缺氧滋养细胞的调节作用。huc-MSCs-EXO 中的 miR-140-5p 通过抑制 FSTL3 来抑制 PE。
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