Zhu Xuanru, Kazemi Aida, Dong Yunqing, Pan Qiao, Jin Panshi, Cheng Biao, Yang Yangog
Department of Burn and Plastic Surgery, General Hospital of Southern Theater Command of PLA, Guangzhou, 510000, China.
Clinical Research Development Unit, Shafa Hospital, Kerman University of Medical Sciences, Kerman, Iran.
J Biomed Nanotechnol. 2022 Feb 1;18(2):535-545. doi: 10.1166/jbn.2022.3249.
In this study we evaluated the impact of topical application of bioactive glass fibers loaded PRP on a deep seconddegree thermal wound and its healing process sub-streaming molecular pathway of re-epithelialization. Wistar rats were randomly divided into four groups: normal control group, model group (deep second-degree thermal wound), PRP group, and PRP+nanobioactive glass fiber group. After treatment, the changes of wounds were observed daily. H&E staining was used to evaluate the pathological changes and also, qRT-PCR was used to detect the mRNA expression of KGF, IL-1, IL-6, IL-10, TGF-, EGF, VEGF, HIF-1, integrin 3 and integrin 1 in wound tissues. In the current study, we observed that PRP group and the PRP group basically re-epithelized on the 21st day. The wound healing rates of the PRP+nanobioactive glass fiber group and PRP group at each time point were higher than those in the model group, while there was no significant difference in wound healing rate between the PRP+nanobioactive glass fiber group and PRP group at each time point. H&E staining showed that the pathological scores of skin wound repairing in the PRP+nanobioactive glass fiber group on the 7th, 14th and 21st day were higher than that of in the model group. The qPCR results suggested the mRNA expression of IL-1, IL-6 and IL-10 in the PRP+nanobioactive glass fiber group and the PRP group were lower than those in the untreated group on the 14th day; the expression of VEGF and EGF mRNA were higher on the 3rd day; the mRNA expression of TGF-, HIF-1 showed a tendency of increasing first and decreasing then; integrin 1 mRNA expression increased significantly, which was highest; integrin 3 mRNA expression was higher on day 3rd and 21th, respectively. The PRP+nanobioactive glass fibers and PRP can shorten the wound healing time and improve the healing quality mainly by promoting the wound epithelization through increasing the expression of EGF, VEGF, TGF-, HIF-1, Integrin 3, and meanwhile increasing the release of Integrin 1 and other mechanisms.
在本研究中，我们评估了局部应用负载富血小板血浆（PRP）的生物活性玻璃纤维对深二度热烧伤创面及其愈合过程中再上皮化分子途径子流的影响。将Wistar大鼠随机分为四组：正常对照组、模型组（深二度热烧伤创面）、PRP组和PRP+纳米生物活性玻璃纤维组。治疗后，每天观察创面变化。采用苏木精-伊红（H&E）染色评估病理变化，同时采用实时定量聚合酶链反应（qRT-PCR）检测创面组织中角质形成细胞生长因子（KGF）、白细胞介素-1（IL-1）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、转化生长因子-β（TGF-β）、表皮生长因子（EGF）、血管内皮生长因子（VEGF）、缺氧诱导因子-1（HIF-1）、整合素β3和整合素α1的mRNA表达。在本研究中，我们观察到PRP组和PRP+纳米生物活性玻璃纤维组在第21天基本实现再上皮化。PRP+纳米生物活性玻璃纤维组和PRP组在各时间点的创面愈合率均高于模型组，而PRP+纳米生物活性玻璃纤维组和PRP组在各时间点的创面愈合率无显著差异。H&E染色显示，PRP+纳米生物活性玻璃纤维组在第7天、第14天和第21天皮肤创面修复的病理评分高于模型组。qPCR结果表明，PRP+纳米生物活性玻璃纤维组和PRP组在第14天时IL-1、IL-6和IL-10的mRNA表达低于未治疗组；第3天时VEGF和EGF mRNA表达较高；TGF-β、HIF-1的mRNA表达呈先升高后降低的趋势；整合素α1 mRNA表达显著增加，且最高；整合素β3 mRNA表达分别在第3天和第21天较高。PRP+纳米生物活性玻璃纤维和PRP主要通过增加EGF、VEGF、TGF-β、HIF-1、整合素β3的表达，同时增加整合素α1的释放等机制促进创面上皮化，从而缩短创面愈合时间，提高愈合质量。
