Department of Mechanical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Sexual Health and Fertility Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
Drug Dev Ind Pharm. 2021 Dec;47(12):1915-1923. doi: 10.1080/03639045.2022.2070640. Epub 2022 May 16.
Solid supports like the extracellular matrix network are necessary for bone cell attachment and start healing in the damaged bone. Scaffolds which are made of different materials are widely used as a supportive structure in bone tissue engineering. In the current study, a 3D polycaprolactone/gelatin bone scaffold was developed by blending electrospinning and freeze-drying techniques for bone tissue engineering. To improve the efficiency of the scaffold, different concentrations of epinephrine (EP) due to its effect on bone healing were loaded. Fabricated scaffolds were characterized by different tests such as surface morphology, FTIR, porosity, compressive strength, water contact angle, and degradation rate. The interaction between prepared scaffolds and blood and cells was evaluated by hemolysis, and MTT test, respectively, and bone healing was evaluated by a rat calvaria defect model. Based on the results, the porosity of scaffolds was about 75% and by adding EP, mechanical strength decreased while due to the hydrophilic properties of it, degradation rate increased. and studies showed the best cell proliferation and bone healing were in PCL/gelatin/EP1% treated group. These results showed the positive effect of fabricated scaffold on osteogenesis and bone healing and the possibility of using it in clinical trials.
固体载体如细胞外基质网络对于骨细胞附着和受损骨的起始愈合是必要的。由不同材料制成的支架被广泛用作骨组织工程中的支撑结构。在本研究中，通过混合静电纺丝和冷冻干燥技术开发了一种 3D 聚己内酯/明胶骨支架，用于骨组织工程。为了提高支架的效率，由于肾上腺素（EP）对骨愈合的影响，加载了不同浓度的 EP。通过不同的测试，如表面形貌、FTIR、孔隙率、压缩强度、水接触角和降解率来表征制备的支架。通过溶血和 MTT 试验分别评估了制备的支架与血液和细胞的相互作用，通过大鼠颅骨缺损模型评估了骨愈合情况。结果表明，支架的孔隙率约为 75%，通过添加 EP，机械强度降低，而由于其亲水性，降解率增加。研究表明，PCL/明胶/EP1%处理组的细胞增殖和骨愈合最好。这些结果表明，所制备的支架对成骨和骨愈合有积极的影响，并有可能在临床试验中使用。
