Biochemistry Laboratory, Research Center, Methodist University Center, Porto Alegre Institute, Porto Alegre, RS, Brazil.
Integraded Colleges of Taquara, Rua Oscar Martins Rangel, Taquara, RS, Brazil.
Birth Defects Res. 2022 Jul 1;114(11):525-535. doi: 10.1002/bdr2.2018. Epub 2022 Apr 29.
Grape juice consumption may influence the early occurrence of ductal constriction during pregnancy, since the consumption of foods rich in polyphenols can be linked to the premature constriction of the ductus arteriosus. This study aimed to evaluate the effect of purple grape juice consumption during gestation on fetal ductus arteriosus closure, prostaglandin levels, and oxidative stress markers in Wistar rats. We divided 18 pregnant rats into four groups: a control group (C), a single-dose grape juice group (SDGJ), a two-dose grape juice group (TDGJ) of 7 μl/g body weight per day, and an indomethacin group (I). Blood was collected on gestational day (GD) 0, 14, and 20. Prostaglandin levels were measured, and the livers and hearts were removed from the mothers and fetuses for oxidative stress analysis; histology of the fetal ductus arteriosus was performed. Prostaglandin levels (pg/ml) at GD 20 were (C:1462.10 ± 314.61); (SDGJ:987.66 ± 86.25); (TDGJ:1290.00 ± 221.57), and (I:584.75 ± 46.77). Fetal ductus arteriosus closure occurred only in the indomethacin group. Lipid peroxidation evaluated through thiobarbituric acid reactive substances (nmol/mg protein) in maternal livers was lower in the grape juice groups (C: 4.11 ± 0.76 nmol/mg protein), (SDGJ: 2.34 ± 0.36), (TDGJ: 1.52 ± 0.18), and (I: 4.20 ± 0.76). Sulfhydryls (nmol/mg protein) were lower in the TDGJ group (C:763.59 ± 61.38 nmol/mg protein), (SDGJ:978.88 ± 158.81), (TDGJ:385.32 ± 86.78), and (I:727.72 ± 49.12). Also, superoxide dismutase activity (USOD/mg protein) was higher in fetal hearts in this group: (C:5.29 ± 0.33), (SDGJ:4.48 ± 0.47), (TDGJ:7.35 ± 0.43), and (I:6.00 ± 0.18). We conclude that grape juice consumption in pregnancy does not induce ductus arteriosus closure in the fetus and presented potential antioxidant effects.
葡萄汁的摄入可能会影响怀孕期间导管的早期收缩，因为摄入富含多酚的食物与动脉导管过早收缩有关。本研究旨在评估妊娠期间摄入紫葡萄汁对胎儿动脉导管闭合、前列腺素水平和 Wistar 大鼠氧化应激标志物的影响。我们将 18 只怀孕的老鼠分为四组：对照组（C）、单次剂量葡萄汁组（SDGJ）、每日 7 μl/g 体重的两次剂量葡萄汁组（TDGJ）和吲哚美辛组（I）。在妊娠第 0、14 和 20 天采集血液。测量前列腺素水平，并从母亲和胎儿中取出肝脏和心脏进行氧化应激分析；对胎儿动脉导管进行组织学检查。妊娠第 20 天的前列腺素水平（pg/ml）为（C：1462.10±314.61）；（SDGJ：987.66±86.25）；（TDGJ：1290.00±221.57）和（I：584.75±46.77）。只有在吲哚美辛组才观察到胎儿动脉导管闭合。通过硫代巴比妥酸反应性物质（nmol/mg 蛋白）评估的母体肝脏脂质过氧化作用在葡萄汁组较低（C：4.11±0.76 nmol/mg 蛋白），（SDGJ：2.34±0.36），（TDGJ：1.52±0.18）和（I：4.20±0.76）。TDGJ 组的硫醇（nmol/mg 蛋白）较低（C：763.59±61.38 nmol/mg 蛋白），（SDGJ：978.88±158.81），（TDGJ：385.32±86.78）和（I：727.72±49.12）。此外，该组胎儿心脏中的超氧化物歧化酶活性（USOD/mg 蛋白）较高：（C：5.29±0.33），（SDGJ：4.48±0.47），（TDGJ：7.35±0.43）和（I：6.00±0.18）。我们得出结论，妊娠期间摄入葡萄汁不会引起胎儿动脉导管闭合，并具有潜在的抗氧化作用。
