Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Zhejiang Engineering Research Center for Biomedical Materials, Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
School of Mechatronics Engineering, Nanchang University, Nanchang 330031, China.
J Mater Chem B. 2022 Jun 8;10(22):4142-4152. doi: 10.1039/d1tb02413b.
Diabetic wounds remain one of the most prevalent hard-to-heal wounds in the clinic. The causative factors impeding the wound healing process include not only the elevated oxidative stress and bacterial infections but also the high and repetitive plantar stress (including compressive pressure and shear stress). Conventional hydrogel dressings are mechanically weak and fragile, limiting their applications in the high stress-loading conditions of diabetic foot ulcers. As such, mechanically tough hydrogel dressings with appropriate bioactivities are highly desirable for diabetic wound treatment. In this study, a mechanically reinforced hydrogel with multiple biofunctionalities was developed a facile and straightforward strategy of incorporation of tannic acid (TA) in zwitterionic poly(sulfobetaine methacrylate) (polySBMA) hydrogel. The polySBMA hydrogel reinforced by TA showed excellent mechanical property, with the tensile stress and compressive stress up to 93.7 kPa and 18.4 MPa, respectively, and it could resist cyclic compressive stress at ∼200 kPa (maximum in-shoe plantar pressure) for up to 3500 cycles. The TA-reinforced zwitterionic hydrogel exhibited strong adhesion to skin tissue (20.2 kPa), which was expected to reduce the shear stress on the foot. The plantar pressure on the foot was significantly reduced by the application of the resilient hydrogel. Attributed to the antioxidant and antibacterial properties of TA, the hydrogel showed rapid radical scavenging capability and strong bactericidal efficacy against Gram-positive and Gram-negative bacteria. and studies confirmed that the hydrogel has good cytocompatibility and negligible skin irritation, and promoted healing of diabetic wounds in mice. Such tough and effective hydrogel with a straightforward preparation strategy holds great promise as wound dressings for diabetic wound treatment.
糖尿病伤口仍然是临床上最常见的难以愈合的伤口之一。阻碍伤口愈合过程的致病因素不仅包括氧化应激和细菌感染增加，还包括高重复性的足底压力（包括压缩压力和剪切力）。传统的水凝胶敷料机械强度弱且易碎，限制了它们在糖尿病足溃疡等高应力加载条件下的应用。因此，具有适当生物活性的机械强度高的水凝胶敷料非常适合用于糖尿病伤口治疗。在这项研究中，开发了一种具有多种生物功能的机械增强水凝胶，这是一种将单宁酸（TA）掺入两性离子聚（磺基甜菜碱甲基丙烯酸酯）（polySBMA）水凝胶中的简便策略。 TA 增强的 polySBMA 水凝胶具有优异的机械性能，拉伸应力和压缩应力分别高达 93.7 kPa 和 18.4 MPa，并且可以抵抗高达 3500 次循环的约 200 kPa（鞋内足底最大压力）的循环压缩应力。TA 增强的两性离子水凝胶对皮肤组织具有很强的附着力（20.2 kPa），这有望减少脚部的剪切力。弹性水凝胶的应用显著降低了足底压力。由于 TA 的抗氧化和抗菌特性，水凝胶表现出快速的自由基清除能力和对革兰氏阳性和革兰氏阴性菌的强杀菌效果。 和 研究证实，水凝胶具有良好的细胞相容性和可忽略不计的皮肤刺激性，并促进了小鼠糖尿病伤口的愈合。这种具有简单制备策略的坚韧有效的水凝胶有望成为治疗糖尿病伤口的伤口敷料。
