Kaminski Pierre-Alexandre
Institut Pasteur, Université de Paris, CNRS UMR2001, Biologie des bactéries pathogènes à Gram-positif, F-75015, Paris, France.
Med Sci (Paris). 2022 Apr;38(4):374-380. doi: 10.1051/medsci/2022041. Epub 2022 Apr 29.
Bacteriophage genomes are the richest source of modified nucleobases of any life form. Of these, 2,6-diaminopurine (2-aminoadénine) that pairs with thymine by forming three hydrogen bonds is the only one violating Watson and Crick's base pairing. 2,6-diaminopurine (2-aminoadénine), initially found in the cyanophage S-2L, is more widespread than expected and has also been detected in bacteriophage infecting Gram-negative and Gram-positive bacteria. The biosynthetic pathway for aminoadenine containing DNA as well as the exclusion of adenine are now elucidated. This example of a natural deviation from the DNA canonical nucleotides represents only one of the possibilities explored by nature and provides a proof of concept for the synthetic biology of non-canonical nucleic acids.
噬菌体基因组是所有生命形式中修饰核碱基最丰富的来源。其中，通过形成三个氢键与胸腺嘧啶配对的2,6-二氨基嘌呤（2-氨基腺嘌呤）是唯一违反沃森和克里克碱基配对规则的。2,6-二氨基嘌呤（2-氨基腺嘌呤）最初在蓝藻噬菌体S-2L中发现，其分布比预期更广，在感染革兰氏阴性菌和革兰氏阳性菌的噬菌体中也有检测到。含氨基腺嘌呤DNA的生物合成途径以及腺嘌呤的排除现已阐明。这种与DNA标准核苷酸自然偏差的例子只是自然界探索的可能性之一，为非标准核酸的合成生物学提供了概念验证。
