Laboratory of Immunology and Cellular and Molecular Biology, Faculty of Chemical Sciences, Autonomous University of San Luis Potosí, UASLP, San Luis Potosí, Mexico.
Unidad de Investigación Biomédica, Delegación Zacatecas, Instituto Mexicano del Seguro Social, IMSS, Interior de la Alameda No.45, 98000, Zacatecas, Zac, México.
Biol Trace Elem Res. 2023 Apr;201(4):1559-1566. doi: 10.1007/s12011-022-03263-x. Epub 2022 Apr 29.
Sodium selenite modulates the activity of lymphocytes. It negatively regulates the suppressive activity of cells and increases the immune response. In this study, we evaluated whether the regulatory T cell differentiation was modulated by sodium selenite. The percentages of CD4+CD25+Foxp3+, CD4+CD25+, and CD4+CTLA-4+ cells in CD4+ T cells cultures stimulated with IL-2 and TGF-β in the presence or absence of selenium, in the form of sodium selenite (2.0×10M), were evaluated by flow cytometry. The mRNA expression of TET2/3 enzymes and IL-10 was analyzed by RT-qPCR and the levels of IL-10 were measured by an ELISA. We observed a decrease in CD4+CD25+Foxp3+ and CD4+CTLA-4+ cells in presence of selenium. However, normal percentages were reached again after selenium removal. An increase in CD4+CTL4-4+ cells was detected in selenium-primed cell cultures in absence of IL-2 and TGF-β. In addition, we observed a decrease in TET3 in presence of selenium. Finally, we observed an augment in IL-10 transcription and protein levels and relative expression of TET2 in cultures exposed to selenium. We suggest that selenium reversibly affects the regulatory T cell differentiation in vitro. Likewise, selenium may modulate Treg percentages promoting optimal immune responses and, at the same time, the expression of specific suppressor molecules.
亚硒酸钠调节淋巴细胞的活性。它负调控细胞的抑制活性，增加免疫反应。在本研究中，我们评估了亚硒酸钠是否调节调节性 T 细胞分化。通过流式细胞术评估了在存在或不存在硒（以亚硒酸钠（2.0×10M）的形式）的情况下，CD4+T 细胞培养物中 IL-2 和 TGF-β刺激下 CD4+CD25+Foxp3+、CD4+CD25+和 CD4+CTLA-4+细胞在 CD4+T 细胞中的百分比。通过 RT-qPCR 分析 TET2/3 酶和 IL-10 的 mRNA 表达，并通过 ELISA 测量 IL-10 水平。我们观察到在存在硒的情况下 CD4+CD25+Foxp3+和 CD4+CTLA-4+细胞减少。然而，在去除硒后，正常百分比再次达到。在缺乏 IL-2 和 TGF-β的情况下，在硒预刺激的细胞培养物中检测到 CD4+CTL4-4+细胞增加。此外，我们观察到在存在硒的情况下 TET3 减少。最后，我们观察到在暴露于硒的培养物中转录和蛋白水平以及 TET2 的相对表达增加。我们认为硒可在体外可逆地影响调节性 T 细胞分化。同样，硒可能调节 Treg 百分比，促进最佳免疫反应，同时表达特异性抑制分子。
