Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
Department of Pathology, The People's Hospital of Honghu, Honghu, Hubei, China.
PLoS One. 2022 Apr 29;17(4):e0267108. doi: 10.1371/journal.pone.0267108. eCollection 2022.
Previous work has demonstrated that the expression of microRNA-21 (miR-21) is implicated in cervical cancer (CC). However, little is known regarding its associations with clinical parameters. We first conducted a meta-analysis using data from Gene Expression Omnibus (GEO) microarrays and The Cancer Genome Atlas (TCGA). Then, enrichment analysis and hub gene screening were performed by bioinformatic methods. Finally, the role of the screened target genes in CC was explored. According to the meta-analysis, the expression of miR-21 in cancer tissues was higher than in adjacent nontumor tissues (P < 0.05). In addition, 46 genes were predicted as potential targets of miR-21. After enrichment analyses, it was detected that these genes were enriched in various cancer pathways, including the phosphatidylinositol signaling system and mammalian target of rapamycin (mTOR) signaling pathway. In this study, bioinformatic tools and meta-analysis validated that miR-21 may function as a highly sensitive and specific marker for the diagnosis of CC, which may provide a novel approach to the diagnosis and treatment of CC.
先前的研究表明，微小 RNA-21（miR-21）的表达与宫颈癌（CC）有关。然而，其与临床参数的关联仍知之甚少。我们首先使用来自基因表达综合数据库（GEO）微阵列和癌症基因组图谱（TCGA）的数据进行了荟萃分析。然后，通过生物信息学方法进行了富集分析和枢纽基因筛选。最后，探讨了筛选出的靶基因在 CC 中的作用。根据荟萃分析，癌组织中 miR-21 的表达高于相邻非肿瘤组织（P<0.05）。此外，预测了 46 个可能是 miR-21 潜在靶标的基因。经过富集分析，发现这些基因富集在各种癌症途径中，包括磷脂酰肌醇信号系统和雷帕霉素（mTOR）信号通路。在这项研究中，生物信息学工具和荟萃分析验证了 miR-21 可能作为 CC 诊断的高度敏感和特异标志物，这可能为 CC 的诊断和治疗提供新的方法。
