Opt Lett. 2022 May 1;47(9):2306-2309. doi: 10.1364/OL.449400.
Studying the basic characteristics of living cells is of great significance in biological research. Bio-physical parameters, including cell-substrate distance and cytoplasm refractive index (RI), can be used to reveal cellular properties. In this Letter, we propose a dual-wavelength surface plasmon resonance holographic microscopy (SPRHM) to simultaneously measure the cell-substrate distance and cytoplasm RI of live cells in a wide-field and non-intrusive manner. Phase-contrast surface plasmon resonance (SPR) images of individual cells at wavelengths of 632.8 nm and 690 nm are obtained using an optical system. The two-dimensional distributions of cell-substrate distance and cytoplasm RI are then demodulated from the phase-contrast SPR images of the cells. MDA-MB-231 cells and IDG-SW3 cells are experimentally measured to verify the feasibility of this approach. Our method provides a useful tool in biological fields for dual-parameter detection and characterization of live cells.
研究活细胞的基本特征在生物研究中具有重要意义。生物物理参数，包括细胞-基底距离和细胞质折射率（RI），可用于揭示细胞特性。在本研究中，我们提出了一种双波长表面等离子体共振全息显微镜（SPRHM），可同时以宽场和非侵入的方式测量活细胞的细胞-基底距离和细胞质 RI。使用光学系统获得单个细胞在 632.8nm 和 690nm 波长下的相衬表面等离子体共振（SPR）图像。然后，从细胞的相衬 SPR 图像中解调细胞-基底距离和细胞质 RI 的二维分布。通过实验测量 MDA-MB-231 细胞和 IDG-SW3 细胞来验证该方法的可行性。我们的方法为生物领域中活细胞的双参数检测和特性分析提供了一种有用的工具。
