Prat M, Corbascio G C, Rossino P, Tusco F, Comoglio P M, Marchisio P C
Basic Appl Histochem. 1986;30(4):417-24.
This paper reports on the production of a monoclonal antibody (i-18) reacting with vimentin, the major structural component of intermediate filaments in cells of mesenchymal origin. The antibody was obtained following immunization with hamster fibroblasts and was selected for its ability to bind to the cytoskeleton fraction of the aforementioned cells. It decorated a perinuclear filamentous network characteristic of vimentin filaments in cells of mesenchymal origin of avian through human species. The specificity of the reagent was further ascertained on the basis of the sensitivity of the decorated filaments to colcemide. The strict antibody specificity for cells of mesenchymal versus epithelial origin was confirmed also in vivo on histological specimens from solid tissue. The i-18 monoclonal antibody precipitated a molecule of about 57 Kd from metabolically labelled cellular extracts. The broad cross-reactivity of this monoclonal antibody among different animal species, as well as its strict in vivo mesenchymal tissue specificity makes this antibody a useful reagent for both experimental and diagnostic purposes.
本文报道了一种与波形蛋白反应的单克隆抗体（i - 18）的产生，波形蛋白是间充质起源细胞中间丝的主要结构成分。该抗体是在用仓鼠成纤维细胞免疫后获得的，并因其与上述细胞的细胞骨架部分结合的能力而被筛选出来。它在鸟类到人类等间充质起源的细胞中勾勒出波形蛋白丝特有的核周丝状网络。基于被标记的丝对秋水仙酰胺的敏感性，进一步确定了该试剂的特异性。在实体组织的组织学标本上，体内实验也证实了该抗体对间充质细胞与上皮细胞起源的严格特异性。i - 18单克隆抗体从经代谢标记的细胞提取物中沉淀出一个约57千道尔顿的分子。这种单克隆抗体在不同动物物种间具有广泛的交叉反应性，以及其在体内对间充质组织的严格特异性，使其成为实验和诊断目的的有用试剂。
