Th1细胞和Th17细胞对T细胞活化诱导的CD6下调具有抗性。
Th1 and Th17 cells are resistant towards T cell activation-induced downregulation of CD6.
作者信息
Brück Carolin, Golumba-Nagy Viktoria, Yan Shuaifeng, Esser Ruth L, Thiele Jan, Stahl David, Pesch Carola Tho, Steinbach-Knödgen Eva, Kofler David M
机构信息
Laboratory of Molecular Immunology, Division of Rheumatology and Clinical Immunology, Department I of Internal Medicine, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany.
Laboratory of Molecular Immunology, Division of Rheumatology and Clinical Immunology, Department I of Internal Medicine, Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany; Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf, Cologne, Germany.
出版信息
Clin Immunol. 2022 May;238:109025. doi: 10.1016/j.clim.2022.109025. Epub 2022 Apr 26.
BACKGROUND
The cell surface molecule CD6 is a modulator of T cell receptor (TCR) signaling. Recently, it has been reported that CD6 is downregulated on CD4+ T cells following T cell activation. This mechanism could limit the efficacy of anti-CD6 therapeutical antibodies.
METHODS
We analyzed CD6 expression on activated and non-activated Th1 cells and Th17 cells by flow cytometry.
RESULTS
Our experiments confirmed a significant downregulation of CD6 on IFNγ- and IL17-negative CD4+ T cells from healthy individuals and from patients with rheumatoid arthritis following T cell activation with anti-CD3 and anti-CD28 antibodies. In contrast, CD6 expression remained stable on activated Th17 cells and Th1 cells.
CONCLUSIONS
Th1 and Th17 cells are resistant towards T cell activation-induced downregulation of CD6. These findings are relevant for the future development of CD6 targeting therapies and show that CD6 expression is differentially regulated in CD4+ T cell subsets.
背景
细胞表面分子CD6是T细胞受体（TCR）信号传导的调节剂。最近，有报道称T细胞活化后CD4⁺T细胞上的CD6表达下调。这种机制可能会限制抗CD6治疗性抗体的疗效。
方法
我们通过流式细胞术分析了活化和未活化的Th1细胞及Th17细胞上的CD6表达。
结果
我们的实验证实，用抗CD3和抗CD28抗体激活T细胞后，健康个体和类风湿性关节炎患者的IFNγ和IL17阴性CD4⁺T细胞上的CD6显著下调。相比之下，活化的Th17细胞和Th1细胞上的CD6表达保持稳定。
结论
Th1和Th17细胞对T细胞活化诱导的CD6下调具有抗性。这些发现与CD6靶向治疗的未来发展相关，并表明CD6表达在CD4⁺T细胞亚群中受到不同调节。
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