Developmental differences in myocardial transmembrane Na transport: implications for excitability and Na handling.

Little is currently known about possible developmental changes in myocardial Na handling, which may have impact on cell excitability and Ca content. Resting intracellular Na concentration ([Na ] ), measured in freshly isolated rat ventricular myocytes with CoroNa green, was not significantly different in neonates (3-5 days old) and adults, but electrical stimulation caused marked [Na ] rise only in neonates. Inhibition of L-type Ca current by CdCl abolished not only systolic Ca transients, but also activity-dependent intracellular Na accumulation in immature cells. This indicates that the main Na influx pathway during activity is the Na /Ca exchanger, rather than voltage-dependent Na current (I ), which was not affected by CdCl . In immature myocytes, I density was two-fold greater, inactivation was faster, and the current peak occurred at less negative transmembrane potential (E ) than in adults. Na channel steady-state activation and inactivation curves in neonates showed a rightward shift, which should increase channel availability at diastolic E , but also require greater depolarization for excitation, which was observed experimentally and reproduced in computer simulations. Ventricular mRNA levels of Na 1.1, Na 1.4 and Na 1.5 pore-forming isoforms were greater in neonate ventricles, while a decrease was seen for the β1 subunit. Both molecular and biophysical changes in the channel profile may contribute to the differences in I density and voltage-dependence, and also to the less negative threshold E , in neonates compared to adults. The apparently lower excitability in immature ventricle may confer protection against the development of spontaneous activity in this tissue. KEY POINTS: Previous studies showed that myocardial preparations from immature rats are less sensitive to electrical field stimulation than adult preparations. Freshly isolated ventricular myocytes from neonatal rats showed lower excitability than adult cells, e.g. less negative threshold membrane potential and greater membrane depolarization required for action potential triggering. In addition to differences in mRNA levels for Na channel isoforms and greater Na current (I ) density, Na channel voltage-dependence was shifted to the right in immature myocytes, which seems to be sufficient to decrease excitability, according to computer simulations. Only in neonatal myocytes did cyclic activity promote marked cytosolic Na accumulation, which was prevented by abolition of systolic Ca transients by blockade of Ca currents. Developmental changes in I may account for the difference in action potential initiation parameters, but not for cytosolic Na accumulation, which seems to be due mainly to Na /Ca exchanger-mediated Na influx.