Leads Rachel R, Magnuson Jason T, Lucero JoAnn, Lund Amie K, Schlenk Daniel, Chavez J Ruben, Roberts Aaron P
University of North Texas, Department of Biological Sciences and Advanced Environmental Research Institute, 1155 Union Circle #305220, Denton, TX 76203, USA.
Department of Environmental Sciences, University of California Riverside, Riverside, CA 92521, USA.
Mar Pollut Bull. 2022 Jun;179:113684. doi: 10.1016/j.marpolbul.2022.113684. Epub 2022 Apr 27.
Ultraviolet (UV) radiation can significantly increase the toxicity of polycyclic aromatic hydrocarbons (PAHs) in crude oil to early life stage (ELS) fishes through photo-induced /photo-enhanced toxicity. However, little is known about the sub-lethal effects and mechanisms of photo-induced PAH toxicity in ELS fishes. The present study investigated apoptosis and global transcriptomic effects in larval red drum (Sciaenops ocellatus) (24-72 h post-fertilization) following co-exposure to oil (0.29-0.30 μg/L ∑PAH) and UV. Apoptosis was quantified using the TUNEL assay, and transcriptomic effects were assessed using RNA sequencing analysis. Apoptotic fluorescence was greatest in the eyes and skin following 24 and 48 h co-exposure to oil and UV, indicating photo-induced toxicity. Consistent with these phenotypic responses, pathways associated with phototransduction, eye development, and dermatological disease were among the top predicted pathways impacted. The present study is the first to provide global transcriptomic analysis of UV and oil co-exposure in an ELS fish.
紫外线（UV）辐射可通过光诱导/光增强毒性显著增加原油中多环芳烃（PAHs）对鱼类早期生活阶段（ELS）的毒性。然而，关于光诱导PAH毒性对ELS鱼类的亚致死效应和机制知之甚少。本研究调查了幼体红鼓鱼（眼斑拟石首鱼）（受精后24 - 72小时）在同时暴露于油（0.29 - 0.30μg/L总PAH）和紫外线后的细胞凋亡及全转录组效应。使用TUNEL检测法定量细胞凋亡，并用RNA测序分析评估转录组效应。在同时暴露于油和紫外线24小时和48小时后，眼睛和皮肤中的凋亡荧光最强，表明存在光诱导毒性。与这些表型反应一致，与光转导、眼睛发育和皮肤病相关的通路是预测受影响最大的前几位通路。本研究首次对ELS鱼类中紫外线和油共同暴露进行了全转录组分析。
