新型激活蛋白-1（AP-1）基因的鉴定及其与文蛤抗弧菌能力的单核苷酸多态性关联分析。
Characterization of a novel activating protein-1 (AP-1) gene and the association of its single nucleotide polymorphisms with vibrio resistance in Tegillarca granosa.
机构信息
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, Zhejiang, 325005, China.
Zhejiang Mariculture Research Institute, Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-Resource, Wenzhou Key Laboratory of Marine Biological Genetics and Breeding, Wenzhou, Zhejiang, 325005, China.
出版信息
Fish Shellfish Immunol. 2022 May;124:552-562. doi: 10.1016/j.fsi.2022.04.023. Epub 2022 Apr 27.
The blood clam Tegillarca granosa is a commercial marine bivalve of economic value, accounting for approximately 50% of clam production in China. In recent years, the yield of blood clams has been threatened by bacterial infections caused by marine Vibrio species that thrive under a rising sea temperature. The transcription factor activating protein-1 (AP-1) is emerging as an important player in the innate immunity of marine bivalves against viral or bacterial infections. In this study, the full-length cDNA of a novel T. granosa AP-1 (TgAP-1) was cloned for the first time. The 1591-bp cDNA encoded a protein of 292 amino acid residues with a calculated molecular weight of 32.8 kDa. The TgAP-1 protein contained an N-terminal Jun domain and a C-terminal basic region leucine zipper domain typically found in Jun proteins (a subfamily of AP-1 proteins). TgAP-1 was ubiquitously expressed in T. granosa, with the highest expression detected in the gill and foot, followed by the mantle, hemolymph, and hepatopancreas. Exposure to Vibrio harveyi induced TgAP-1 expression in gill tissues and the expression levels of TgAP-1 of resistant blood clams were always lower than that of control population whether Vibro infection or not. A total of 18 single nucleotide polymorphisms (SNPs) of TgAP-1 were detected in T. granosa. SNP-typing and haplotyping of resistant and susceptible populations revealed that six SNPs (AG type of TgSNP-1, GA type of TgSNP-2, TG type of TgSNP-4, CT type of TgSNP-7, AG type of TgSNP-11, and GA type of TgSNP-12) and four haplotypes (fHap2, fHap3, fHap6, and fHap7) were significantly associated with V. harveyi resistance. Risk assessment showed that fHap2 (CG) and fHap7 (GA) were associated with an increased resistance, while fHap3 (CT) and fHap6 (AG) were associated with an increased susceptibility. The results from this study supported a potential role of TgAp-1 in the anti-Vibro immunity of T. granosa. The discovery of the genetic molecular markers and haplotypes related to Vibrio resistance can provide guidance for selective breeding of T. granosa in the future.
血蛤（Tegillarca granosa）是一种具有经济价值的商业性海洋双壳贝类，约占中国贝类产量的 50%。近年来，由于海洋弧菌（Vibrio）在海水温度升高的情况下大量繁殖，导致血蛤的产量受到细菌性感染的威胁。转录因子激活蛋白-1（AP-1）作为海洋双壳贝类抗病毒或细菌感染固有免疫的重要参与者而崭露头角。本研究首次克隆了一种新型 T. granosa AP-1（TgAP-1）的全长 cDNA。该 1591bp 的 cDNA 编码一个 292 个氨基酸残基的蛋白质，预测分子量为 32.8 kDa。TgAP-1 蛋白含有一个 N 端 Jun 结构域和一个 C 端碱性亮氨酸拉链结构域，通常存在于 Jun 蛋白（AP-1 蛋白的一个亚家族）中。TgAP-1 在 T. granosa 中广泛表达，在鳃和足部的表达量最高，其次是套膜、血淋巴和肝胰腺。Vibrio harveyi 暴露后，TgAP-1 在鳃组织中表达诱导，并且无论是否存在 Vibro 感染，抗性血蛤的 TgAP-1 表达水平始终低于对照种群。在 T. granosa 中检测到了 18 个 TgAP-1 的单核苷酸多态性（SNP）。对抗性和易感种群的 SNP 分型和单体型分析表明，有 6 个 SNP（TgSNP-1 的 AG 型、TgSNP-2 的 GA 型、TgSNP-4 的 TG 型、TgSNP-7 的 CT 型、TgSNP-11 的 AG 型和 TgSNP-12 的 GA 型）和 4 种单体型（fHap2、fHap3、fHap6 和 fHap7）与抗 V. harveyi 显著相关。风险评估表明，fHap2（CG）和 fHap7（GA）与抗性增加相关，而 fHap3（CT）和 fHap6（AG）与易感性增加相关。本研究结果支持 TgAp-1 在 T. granosa 抗 Vibrio 免疫中的潜在作用。发现与抗弧菌相关的遗传分子标记和单体型可为今后 T. granosa 的选择性育种提供指导。
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