Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, Paraná, Brazil.
Programa de Pós-Graduação em Ciências Veterinárias, Universidade Estadual do Centro-Oeste, 85040-080 Guarapuava, Paraná, Brazil; Embrapa Suínos e Aves, 89715-899 Concórdia, Santa Catarina, Brazil.
Res Vet Sci. 2022 Oct;147:92-95. doi: 10.1016/j.rvsc.2022.04.006. Epub 2022 Apr 25.
Femoral head necrosis (FHN) is one of the most common conditions in fast growing broilers, being characterized by separation of articular cartilage from epiphysis and classified as femoral head separation (FHS) or FHS with laceration (FHSL) depending on severity. Although molecular mechanisms involved with this disorder have been observed, its etiology is still unclear. Therefore, the expression of 15 candidate genes, chosen based on previous transcriptomic studies, was evaluated in the articular cartilage (AC) of normal and FHS-affected broilers at 21 days of age. Samples were collected based on the absence or presence of FHS for physical-chemical and qPCR analysis. The AvBD2, RHAG, COL28A1, ADA and ANGPTL7 were upregulated in FHS-affected broilers compared to the healthy group. These genes are involved in immune response, defense against pathogens, inflammation, cellular migration and adhesion, indicating different molecular mechanisms to control FHS progression at early age. Our results can contribute to improve the knowledge on FHN etiology in chickens and other species, such as horse and pigs that are severely affected by bone disorders.
股骨头坏死（FHN）是快速生长的肉鸡中最常见的病症之一，其特征是关节软骨与骨骺分离，并根据严重程度分为股骨头分离（FHS）或伴有撕裂的 FHS（FHSL）。尽管已经观察到与这种疾病相关的分子机制，但它的病因仍不清楚。因此，根据先前的转录组研究，选择了 15 个候选基因，在 21 日龄时正常和受 FHS 影响的肉鸡的关节软骨（AC）中评估了这些基因的表达情况。根据是否存在 FHS 进行采样，用于理化分析和 qPCR 分析。与健康组相比，受 FHS 影响的肉鸡中 AvBD2、RHAG、COL28A1、ADA 和 ANGPTL7 的表达上调。这些基因参与免疫反应、抵御病原体、炎症、细胞迁移和黏附，表明在早期控制 FHS 进展有不同的分子机制。我们的研究结果有助于提高对鸡和其他物种（如马和猪）FHN 病因的认识，这些物种的骨骼疾病严重。
