Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 613000, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 613000, China.
Poult Sci. 2022 Jun;101(6):101819. doi: 10.1016/j.psj.2022.101819. Epub 2022 Mar 12.
The light intensity can affect the production performance of animals. The retina and pineal gland, closely linked, are directly photosensitive organs. This study evaluated the effect of light intensity on duck growth and investigated the effects of varying light intensities on retina and pineal gland transcriptome changes. The increase of light intensity will significantly decrease production performance, such as body weight, eviscerated weight, breast muscle weight, percentage of abdominal fat, etc. The RNA-seq revealed 967 and 201 differentially expressed genes (DEGs) in the retina and pineal gland under different light intensities, respectively. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) in the retina showed the DEGs were enriched in ECM-receptor interaction, Focal adhesion, Cell adhesion molecules (CAMs), Cytokine-cytokine receptor interaction, Melanogenesis, etc. Meanwhile, the DEGs in the pineal gland were mainly enriched in pathways associated with the mTOR signaling pathway, melanin production pathway, etc. Our results indicated that different light intensities might affect the function of the retina and pineal gland, including the melanin production of the retina and the secretion of melatonin in the pineal gland. Our study can provide a theoretical basis for the molecular mechanism of the effects of different light intensities on the retina and pineal gland.
光照强度会影响动物的生产性能。紧密相连的视网膜和松果体是直接感光器官。本研究评估了光照强度对鸭生长的影响，并研究了不同光照强度对视网膜和松果体转录组变化的影响。光照强度的增加会显著降低生产性能，如体重、屠体重量、胸肌重量、腹部脂肪百分比等。RNA-seq 显示，在不同光照强度下，视网膜和松果体中的差异表达基因（DEGs）分别有 967 和 201 个。视网膜中的基因本体论（GO）和京都基因与基因组百科全书（KEGG）显示，DEGs 在细胞外基质-受体相互作用、黏附斑、细胞黏附分子（CAMs）、细胞因子-细胞因子受体相互作用、黑色素生成等途径中富集。同时，松果体中的 DEGs 主要富集在与 mTOR 信号通路、黑色素生成途径等相关的途径中。我们的结果表明，不同的光照强度可能会影响视网膜和松果体的功能，包括视网膜的黑色素生成和松果体中褪黑素的分泌。我们的研究可为不同光照强度对视网膜和松果体影响的分子机制提供理论依据。
