Qiu Peng, Bi Jiancheng, Liu Jia, Lai Chaohui, Li Xiaoquan
Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, China.
Medicine Medical Laboratory, Hebei Provincial Hospital of Traditional Chinese Medicine, Shijiazhuang, Hebei 050011, China.
Pathol Res Pract. 2022 Jun;234:153884. doi: 10.1016/j.prp.2022.153884. Epub 2022 Apr 2.
Pituitary adenoma (PA) is a common intracranial tumor, and its incidence has been on the rise in recent years. Pituitary tumor not only causes intracranial space occupying signs, but also produces endocrine disorders, such as infertility, sexual dysfunction, facial and limb changes. Moreover, it destroys the internal environment stability and even affects the appearance of a person. However, the mechanism of PA is not fully understood. Previous research has confirmed that the expression or role of long non-coding RNA is closely connected with the occurrence of human diseases. In this study, we discovered that long intergenic non-protein coding RNA 1004 (LINC01004) was aberrantly up-regulated in PA cells. Functional assays manifested that LINC01004 promoted malignant behaviors of PA cells in vitro and growth of PA in vivo. By using bioinformatics tools and a series of mechanism assays, LINC01004 was identified to sponge miR-323a-3p/miR-136-5p to enhance the expression of RCN2 in PA. In conclusion, the results provided in this study revealed a novel regulatory mechanism of LINC01004 in PA, which might be helpful for the treatment of PA and supply a new thought for further research of PA.
垂体腺瘤（PA）是一种常见的颅内肿瘤，近年来其发病率呈上升趋势。垂体瘤不仅会引起颅内占位征象，还会导致内分泌紊乱，如不孕、性功能障碍、面部及肢体变化。此外，它会破坏内环境稳定，甚至影响人的外貌。然而，PA的发病机制尚未完全明确。既往研究证实，长链非编码RNA的表达或作用与人类疾病的发生密切相关。在本研究中，我们发现长链基因间非编码RNA 1004（LINC01004）在PA细胞中异常上调。功能实验表明，LINC01004在体外促进PA细胞的恶性行为，在体内促进PA的生长。通过生物信息学工具和一系列机制实验，确定LINC01004可吸附miR-323a-3p/miR-136-5p，从而增强PA中RCN2的表达。总之，本研究结果揭示了LINC01004在PA中的一种新的调控机制，这可能有助于PA的治疗，并为PA的进一步研究提供新的思路。
