Department of Urology, Soonchunhyang University Seoul Hospital, Soonchunhyang University Medical College, Seoul, Korea.
Department of Pharmacology, 37977Ajou University School of Medicine, Suwon, Republic of Korea.
Technol Cancer Res Treat. 2022 Jan-Dec;21:15330338221093146. doi: 10.1177/15330338221093146.
It has been proposed that CRPC treatment with reduced systemic toxicity can be achieved by employing genes that express enzymes that activate pharmacological agents. In this paper, we report our study that used human adipose-derived stem cells (ADSC), rabbit CE, and human TRAIL with reduced toxicity to explore how tumor development can be suppressed in CRPC-bearing mouse models. and directional migration of ADSC.CE.sTRAIL cells toward PC3 cells was significantly stimulated.ADSC.CE.sTRAIL showed higher suicide effects than did ADSC, ADSC.CE, or ADSC.sTRAIL under CPT-11 treatment. PC3 cells co-cultured with ADSC.CE.TRAIL showed higher cytotoxicity than did CPT-11 monotherapy, ADSC.CE, or ADSC.sTRAIL under CPT-11 treatment. ADSC.CE.sTRAIL showed higher apoptosis than did CPT-11 monotherapy, ADSC.CE, or ADSC.sTRAIL under CPT-11 treatment. In the study, ADSC.CE.sTRAIL inhibited tumor growth more than did CPT-11 monotherapy, ADSC.CE, or ADSC.sTRAIL under CPT-11 treatment. The evidence suggests that patients' own ADSC could be used in clinical trials for CRPC treatment based on therapeutic stem cells that express CE and TRAIL complex genes.
有人提出，通过使用表达能激活药物的酶的基因，可以实现降低毒性的 CRPC 治疗。在本文中，我们报告了一项研究，该研究使用了人脂肪来源的干细胞（ADSC）、兔 CE 和 TRAIL，降低了毒性，以探索如何在患有 CRPC 的小鼠模型中抑制肿瘤发展。ADSC.CE.sTRAIL 细胞对 PC3 细胞的定向迁移显著受到刺激。与 CPT-11 治疗相比，ADSC.CE.sTRAIL 显示出更高的自杀效应，而 ADSC、ADSC.CE 或 ADSC.sTRAIL 则没有。与 CPT-11 单药治疗、ADSC.CE 或 ADSC.sTRAIL 相比，共培养的 PC3 细胞显示出更高的细胞毒性。与 CPT-11 单药治疗、ADSC.CE 或 ADSC.sTRAIL 相比，ADSC.CE.sTRAIL 显示出更高的细胞凋亡。在这项研究中，ADSC.CE.sTRAIL 抑制肿瘤生长的效果优于 CPT-11 单药治疗、ADSC.CE 或 ADSC.sTRAIL。有证据表明，基于表达 CE 和 TRAIL 复合物基因的治疗性干细胞，患者自身的 ADSC 可用于 CRPC 治疗的临床试验。
