强直性肌营养不良症患者肌肉培养物中的蛋白质合成。A23187离子载体和钙的影响：初步研究。

Protein synthesis in muscle cultures from patients with myotonic dystrophy. Influence of A23187 ionophore and calcium: preliminary investigation.

作者信息

Ionasescu V, Ionasescu R, Cancilla P, Kahn L, Zellweger H

出版信息

Eur Neurol. 1977;16(1-6):155-60. doi: 10.1159/000114894.

Abstract

Muscle samples for cultures were obtained from the tibialis anterior by open biopsy under local anesthesia in 12 patients with myotonic dystrophy and 15 controls. Total protein synthesis in muscle cultures from patients with myotonic dystrophy showed a nonsignificant increase in (3H)-leucine incorporation. Addition of A23187 ionophore significantly stimulated the protein synthesis in muscle cultures from patients with myotonic dystrophy, but had an inhibitory effect in the cultures from controls. Myosin heavy chain synthesis was measured and found normal in all patients with myotonic dystrophy.

摘要

在局部麻醉下，通过开放性活检从12例强直性肌营养不良患者和15例对照者的胫前肌获取用于培养的肌肉样本。强直性肌营养不良患者肌肉培养物中的总蛋白质合成显示，（3H）-亮氨酸掺入量有不显著的增加。添加A23187离子载体可显著刺激强直性肌营养不良患者肌肉培养物中的蛋白质合成，但对对照者的培养物有抑制作用。对肌球蛋白重链合成进行了测量，发现所有强直性肌营养不良患者的该项指标均正常。