School of Pharmacy, Linyi University, Linyi, Shandong, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Front Cell Infect Microbiol. 2022 Apr 13;12:855920. doi: 10.3389/fcimb.2022.855920. eCollection 2022.
Genome rearrangement occurs to porcine circovirus type 2 (PCV2) during and infections, and a number of rearranged PCV2 genomes have been isolated and characterized. This study was conducted to investigate the role of the rearranged PCV2 (rPCV2) in PCV2 replication and the biological effect of rPCV2 in host cells. Two whole rPCV2 genome sequences (358 nt and 1125 nt in length) were synthesized and recombinant plasmids pBSK(+)-rPCV2 (pBSK(+)-1125 and pBSK(+)-358) were constructed. A novel virus-like agent (rPCV2-1125) was rescued by transfection of porcine kidney cell line (PK-15) and porcine alveolar macrophage 3D4/21 cells. The data indicate that rPCV2-1125 significantly enhanced PCV2 replication . Furthermore, rPCV2-1125 led to oxidative stress in host cells, as indicated by decreased intracellular glutathione (GSH) and total superoxide dismutase (SOD) activities, as well as increased malondialdehyde (MDA) levels. These results provide new insights into genome rearrangement of PCV2 and will contribute to future studies of PCV2 replication and associated mechanisms.
猪圆环病毒 2 型（PCV2）在 和 感染期间会发生基因组重排，并且已经分离和鉴定了许多重排的 PCV2 基因组。本研究旨在探讨重排的 PCV2（rPCV2）在 PCV2 复制中的作用以及 rPCV2 在宿主细胞中的生物学效应。合成了两个全长 rPCV2 基因组序列（长度为 358nt 和 1125nt），并构建了重组质粒 pBSK(+)-rPCV2（pBSK(+)-1125 和 pBSK(+)-358）。通过转染猪肾细胞系（PK-15）和猪肺泡巨噬细胞 3D4/21 细胞，拯救出了一种新型病毒样颗粒（rPCV2-1125）。数据表明，rPCV2-1125 显著增强了 PCV2 的复制 。此外，rPCV2-1125 导致宿主细胞发生氧化应激，表现为细胞内谷胱甘肽（GSH）和总超氧化物歧化酶（SOD）活性降低，以及丙二醛（MDA）水平升高。这些结果为 PCV2 基因组重排提供了新的见解，并将有助于未来对 PCV2 复制及其相关机制的研究。
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