Chu Lanling, Wang Yunzheng, Zhou Yu, Kang Xuejun
School of Light Industry and Food Engineering, Nanjing Forestry University Nanjing Jiangsu 210037 China.
School of Biological Science and Medical Engineering, Southeast University Nanjing Jiangsu 210096 China
RSC Adv. 2021 Dec 13;11(63):39666-39671. doi: 10.1039/d1ra07061d.
Biosensor substrate materials are a key research focus in the field of sensors. Blu-ray discs (BDs) as universal sensor substrates are advantageous in comparison with other substrates (conventional paper and polycarbonate) in terms of easier activity treatment and higher density of reactive groups on the film surface. In this study, a novel and simple microfluidic biosensor based on BD coating film was developed by treating with sodium hydroxide solution and a piece of filter paper at slightly elevated temperatures. There are no significant physical damages to the substrate morphology, and the aging effect is minimal. The unique wetting, optical, and self-cleaning properties of the modified surfaces can be demonstrated in the paper. We have tested this new type of biosensor substrates for assay applications (the determination of total amino acids in tea leaves), which showed excellent performance in terms of sensitivity and reproducibility. The novel biosensor substrate material based on a simple BD coating film displayed preferable merits with easy making, low cost, easy using, and extensive application prospect.
生物传感器基底材料是传感器领域的一个关键研究重点。蓝光光盘（BD）作为通用传感器基底，与其他基底（传统纸张和聚碳酸酯）相比，在活性处理更容易以及薄膜表面反应基团密度更高方面具有优势。在本研究中，通过在略高温度下用氢氧化钠溶液和一张滤纸处理，开发了一种基于BD涂层薄膜的新型简易微流控生物传感器。基底形态没有明显的物理损伤，老化效应最小。本文可以证明改性表面独特的润湿性、光学性和自清洁性能。我们已经测试了这种新型生物传感器基底用于分析应用（测定茶叶中的总氨基酸），其在灵敏度和重现性方面表现出优异的性能。基于简单BD涂层薄膜的新型生物传感器基底材料具有制作简便、成本低、使用方便和广泛的应用前景等优点。
