The Correlation of Chronological Age and Micro Ribonucleic Acid-135b Expression in Spent Culture Media of Fertilisation Patient.

BACKGROUND

The lack of accuracy in embryo viability assessment methods still remains a challenge to increase the fertilisation (IVF) success rate. The chronological age and micro ribonucleic acid (RNA)-135b influence the quality of the embryo since microRNA-135b expresses stably in the spent culture media. Therefore, microRNA-135b has the potential to become a non-invasive biomarker of IVF embryo quality.

AIMS

(1) The aim of this study is to determine the chronological age and microRNA-135b expression distribution of IVF patients. (2) to determine the correlation between chronological age and microRNA-135b expression in spent culture media of IVF patients.

STUDY SETTING AND DESIGN

An observational study was conducted in Yasmin IVF clinic.

MATERIALS AND METHODS

The chronological age data were collected from the medical records and 31 spent culture media samples from 11 IVF patients were taken on day 5 of embryo culture. We also collected the basal media sample as the control group. The microRNA-135b expression was analysed using quantitative real-time polymerase chain reaction (qPCR) analysis.

STATISTICAL ANALYSIS USED

The data analysis was performed using IBM SPSS Statistics 25.

RESULTS

The chronological age and microRNA-135b expression were distributed abnormally. There was a significant positive correlation with moderate statistical power between chronological age and microRNA-135b expression in spent culture media. MicroRNA-135b expression increased 4.9-fold in spent culture media than basal media of IVF.

CONCLUSIONS

The increase of chronological age is followed by the rise of microRNA-135b expression in spent culture media of IVF patients. The microRNA-135b is a potential biomarker to predict IVF embryo quality.