The cellular location of 1 alpha-hydroxycorticosterone binding protein in skate.

Antiserum to 1 alpha-hydroxycorticosterone binding protein was used to investigate its location in selected tissues of the skate Raja ocellata. Immunofluorescence, using an indirect technique, showed 1 alpha-hydroxycorticosterone binding sites in potential target tissue: gill. chloride cells, rectal gland parenchyma, and a portion of the kidney tubule. The binding protein was not detectable in the ventricle or the intestinal spiral valve but was associated with liver parenchyma and interrenal cells. The intracellular location of the binding protein is apparently tissue specific.