Boudbid H, Leger A F, Pidoux E, Volle G E, Taboulet J, Moukhtar M S, Treilhou-Lahille F
Gen Comp Endocrinol. 1987 Mar;65(3):415-22. doi: 10.1016/0016-6480(87)90127-4.
Immunohistochemical methods using affinity adsorbed antibodies raised against the three families of calcitonins (CT) were applied to ultimobranchial (UB) cells in situ to investigate the nature of the Chelonian calcitonin molecule and its distribution in the ultimobranchial bodies of the freshwater turtle, Pseudemys scripta. In this species, the UB glands were present on both sides and consisted of scattered cell clumps between epithelial vesicular structures. The neighboring parathyroid tissue also contained two components, the majority being composed of similar vesicles, with occasional solid cell cords evenly distributed. Calcitonin immunoreactivity was found in the cell clumps of the UB gland and in the cell cords of the associated parathyroid, but not in the epithelial component lining the vesicles or in the amorphous material which sometimes filled the lumen. Turtle calcitonin was exclusively of the salmon type, as determined by the negative results obtained in situ after the use of antibodies raised against human and porcine molecules. The salmon-like calcitonin content of the ultimobranchial area was estimated as 15.2 ng; however, the molecule was undetectable in the circulation. In this work we localize the quantitate a salmon-like CT molecule in one type of ultimobranchial and parathyroid cell of a reptile for the first time.
运用针对三种降钙素（CT）家族产生的亲和吸附抗体的免疫组织化学方法，对龟类最后鳃体（UB）细胞进行原位研究，以探究龟降钙素分子的性质及其在淡水龟（伪龟）最后鳃体中的分布。在该物种中，双侧均存在UB腺，由上皮泡状结构之间散在的细胞团组成。相邻的甲状旁腺组织也包含两个成分，大部分由类似的泡状结构组成，偶尔有散在分布的实性细胞索。在UB腺的细胞团以及相关甲状旁腺的细胞索中发现了降钙素免疫反应性，但在泡状结构内衬的上皮成分或有时填充管腔的无定形物质中未发现。通过使用针对人和猪分子产生的抗体原位检测得到的阴性结果确定，龟降钙素完全是鲑鱼型。最后鳃体区域的鲑鱼样降钙素含量估计为15.2纳克；然而，在循环中未检测到该分子。在这项工作中，我们首次在一种爬行动物的一种最后鳃体和甲状旁腺细胞中定位并定量了一种鲑鱼样CT分子。
