Waters L S, Taverne J, Tai P C, Spry C J, Targett G A, Playfair J H
Infect Immun. 1987 Apr;55(4):877-81. doi: 10.1128/iai.55.4.877-881.1987.
The multiplication of two strains of Plasmodium falciparum in culture, as measured by [3H]hypoxanthine incorporation, was inhibited in a dose-dependent manner by granule proteins secreted by purified eosinophils obtained from patients with the hypereosinophilic syndrome. Morphological examination revealed the presence of abnormal parasites inside erythrocytes, indicating that they were killed in situ, and the later stages of the developmental cycle were found to be most susceptible to these toxic effects. A monoclonal antibody against eosinophil cationic protein partially blocked the inhibitory effect, suggesting that it was caused by more than one of the eosinophil granule proteins. Thus some of the antimalarial effects of molecules such as the tumor necrosis factor, which activates eosinophils, may be mediated through the enhanced production of eosinophil secretion products.
用[3H]次黄嘌呤掺入法测定，嗜酸性粒细胞增多综合征患者纯化嗜酸性粒细胞分泌的颗粒蛋白可剂量依赖性抑制两种恶性疟原虫在培养中的增殖。形态学检查显示红细胞内存在异常寄生虫，表明它们在原位被杀死，并且发现发育周期的后期对这些毒性作用最敏感。一种针对嗜酸性粒细胞阳离子蛋白的单克隆抗体部分阻断了抑制作用，提示这是由多种嗜酸性粒细胞颗粒蛋白引起的。因此，一些激活嗜酸性粒细胞的分子如肿瘤坏死因子的抗疟作用可能是通过增强嗜酸性粒细胞分泌产物的产生来介导的。
