Protocol for lysine deacetylation to test putative substrates of class III deacetylases.

Lysine acetylation is an important post-translational modification that is used in multiple cellular pathways, such as the regulation of gene expression at the histone level. The purpose of this assay is to test for putative substrates of class III deacetylases using an method. The analysis helps circumvent confounding variables when assessing for a direct relationship between deacetylase and substrate, such as the effects of other cellular deacetylases or acetyltransferases that modify the substrate . For complete details on the use and execution of this protocol, please refer to Minten et al. (2021).