人视网膜神经节细胞在鼠器官型视网膜培养物中移植后的形态和定位分析。
Analyses of transplanted human retinal ganglion cell morphology and localization in murine organotypic retinal explant culture.
机构信息
Glaucoma Center of Excellence, Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 21287, USA.
出版信息
STAR Protoc. 2022 Apr 18;3(2):101328. doi: 10.1016/j.xpro.2022.101328. eCollection 2022 Jun 17.
Retinal ganglion cell (RGC) transplantation has the potential to restore vision in optic neuropathy, but donor neuron survival and retinal integration remain challenging. Here, we present a protocol for human RGC transplantation on flatmounted murine organotypic retinal explants, providing a robust platform for studying donor RGC survival, dendritic stratification, topographic distribution, donor-host interactions, and pro-engraftment strategies. The protocol includes microscopy-based analyses to evaluate donor cell engraftment and can be adapted to various donor cell types or culture systems. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2021a, 2021b).
视网膜神经节细胞（RGC）移植有可能恢复视神经病变的视力，但供体细胞的存活和视网膜整合仍然具有挑战性。本研究提供了一种在平铺的鼠类器官型视网膜外植体上进行人 RGC 移植的方案，为研究供体 RGC 的存活、树突分层、拓扑分布、供体-宿主相互作用以及移植前策略提供了一个强大的平台。该方案包括基于显微镜的分析，用于评估供体细胞的植入情况，并可适应各种供体细胞类型或培养系统。如需了解该方案的详细使用和执行情况，请参考 Zhang 等人（2021a，2021b）。
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