评估基因在保护小鼠视网膜神经节细胞中的作用的方案。
Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells.
机构信息
Departments of Ophthalmology and Neuroscience, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
出版信息
STAR Protoc. 2021 Nov 3;2(4):100932. doi: 10.1016/j.xpro.2021.100932. eCollection 2021 Dec 17.
The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).
视网膜神经节细胞（RGCs）的变性会导致多种病理状态下的视力不可逆转丧失。在这里，我们描述了一种评估基因在保护小鼠 RGCs 免受兴奋性毒性或轴突损伤时作用的方案。该方案包括通过 AAV 玻璃体内注射进行基因转移的程序、通过 NMDA 诱导的兴奋性毒性或视神经挤压诱导 RGC 损伤，以及评估 RGC 存活的视网膜免疫组织化学。有关此方案的使用和执行的完整详细信息，请参阅 Guo 等人。（2021 年）。
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