Hegseth Tristan, Lee Bryan, AuCoin David, Zhu Xiaoshan
Department of Electrical and Biomedical Engineering, University of Nevada Reno, NV 98557 USA.
Department of Microbiology and Immunology, University of Nevada Reno, NV 89557 USA.
IEEE Sens Lett. 2022 Apr;6(4). doi: 10.1109/lsens.2022.3159761. Epub 2022 Mar 16.
Time-resolved fluorescence measurement, a technique utilized to improve sensitivity for fluorescence detection, is becoming more accessible due to the recent development of bright nanolabels with long fluorescence lifetimes and low-energy excitation. Currently, instruments taking advantage of this technique for broader applications (in-field or point-of-care testing) are lagging behind in their development. In this work, a low-cost, compact, and sensitive time-resolved-optical-reader was developed aiming to advance such a technique for wider applications.
时间分辨荧光测量是一种用于提高荧光检测灵敏度的技术，由于最近具有长荧光寿命和低能量激发的明亮纳米标签的发展，该技术正变得越来越容易获得。目前，利用这种技术进行更广泛应用（现场或即时检测）的仪器在其开发方面滞后。在这项工作中，开发了一种低成本、紧凑且灵敏的时间分辨光学阅读器，旨在推动这种技术的更广泛应用。
