Pan Yanfang, Chen Lan, Pang Lingling, Chen Xiaotong, Jia Xiaoyu, Li Xihong
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology Tianjin 300457 China
Tianjin Gasin-DH Preservation Technology Co., Ltd Tianjin 300300 China.
RSC Adv. 2020 Mar 3;10(16):9193-9202. doi: 10.1039/c9ra06418d. eCollection 2020 Mar 2.
Controlling browning and mitigating oxidative damage are important factors when attempting to extend the shelf-life and high-quality features of fresh-cut sweet potato ( (L.) Lam). In order to preserve the color and antioxidant capacity, ultrasound (US) treatment at 40 kHz for 10 min was applied to investigate the effect on enzymatic browning of sweet potato slices. Changes in color, total phenolic content, total antioxidant capacity, phenol metabolism-related enzymes including phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD) were examined. Also investigated here were superoxide radical (O ˙) and hydrogen peroxide (HO) contents, antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) involved in reactive oxygen species metabolism. After storage lasting 10 days at 4 °C, US-treated slices maintained significantly ( < 0.05) higher luminosity ( = 0.000003) and chroma ( = 0.000018) by reducing PPO and POD activities, when compared to the control. Meanwhile, the induction of PAL was observed to positively correlate with higher total phenolic content ( = 0.818, < 0.01; = 6.1752 × 10), thereby enhancing antioxidant capacity to combat oxidative damage. Moreover, O ˙ ( = 3.8046 × 10) and HO ( = 0.000013) concentrations were significantly ( < 0.05) suppressed by activating CAT and SOD activities. Results suggested that US treatment could inhibit browning of fresh-cut sweet potato by reducing the activity of PPO and POD while improving total antioxidant capacity.
在试图延长鲜切甘薯(Ipomoea batatas (L.) Lam)的货架期和保持其高品质特性时,控制褐变和减轻氧化损伤是重要因素。为了保持颜色和抗氧化能力,采用40 kHz超声波处理10分钟,以研究其对甘薯切片酶促褐变的影响。检测了颜色、总酚含量、总抗氧化能力、与酚类代谢相关的酶(包括苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)、过氧化物酶(POD))的变化。还研究了超氧自由基(O˙)和过氧化氢(H₂O₂)的含量,以及参与活性氧代谢的抗氧化酶(包括超氧化物歧化酶(SOD)和过氧化氢酶(CAT))。在4℃下储存10天后,与对照相比,经超声处理的切片通过降低PPO和POD活性,显著(P<0.05)保持了更高的亮度(P = 0.000003)和色度(P = 0.000018)。同时,观察到PAL的诱导与较高的总酚含量呈正相关(r = 0.818,P<0.01;P = 6.1752×10),从而增强了对抗氧化损伤的抗氧化能力。此外,通过激活CAT和SOD活性,显著(P<0.05)抑制了O˙(P = 3.8046×10)和H₂O₂(P = 0.000013)的浓度。结果表明,超声处理可通过降低PPO和POD的活性,同时提高总抗氧化能力,抑制鲜切甘薯的褐变。