Yang Shuopeng, Han Yanzhen, Wang Kairui, Wang Yu, Li Liping, Li Nan, Xu Xiangdong
School of Public Health, Key Laboratory of Environment and Human Health of Hebei Medical University Shijiazhuang 050017 China
Tianjin Center for Disease Control and Prevention Tianjin 300011 China.
RSC Adv. 2021 Oct 20;11(54):33996-34003. doi: 10.1039/d1ra06608k. eCollection 2021 Oct 18.
Chlorogenic, ferulic, vanillic, and caffeic acids are phenolic acids found in natural drugs. They possess the biological activities of scavenging free radicals and inhibiting thrombus formation. Phenolic acids can inhibit the oxidation of low-density lipoprotein, as well as have anti-inflammatory effects. This paper reports for the first time a capillary electrophoresis-chemiluminescence (CE-CL) method for the simultaneous determination of the four phenolic acids found in traditional and proprietary Chinese medicine, including Miller, Shuanghuanglian oral liquid, and granules. Capillary electrophoretic separation was performed on a self-assembled CE-CL device with an uncoated fused-silica capillary (66 cm effective length, 50 μm i.d.), and the background electrolyte was composed of 3.0 × 10 M Ag(iii) (pH = 12.01), 3.0 mM luminol (pH = 9.20), and 10 mM sodium tetraborate solution. The injection time was 12 s (under gravity) and the separation voltage was 22 kV. The combination of solid-phase extraction (SPE) and CE-CL improves the sensitivity. Under optimal conditions, calibration graphs displayed a linear range between 0.625 and 20.0, 1.000 and 30.0, 0.150 and 1.50, and 0.045 and 1.00 μg mL for chlorogenic, ferulic, vanillic, and caffeic acid, respectively. The detection limit ranged from 0.014 to 0.300 μg mL. The practicality of using the proposed method to determine the four target analytes in traditional Chinese medicine was also validated, in which recoveries ranged from 90.9% to 119.8%. Taken together, these results indicate that the developed method is sensitive and reliable. Furthermore, the method was successfully applied to real traditional Chinese medicine samples.
绿原酸、阿魏酸、香草酸和咖啡酸是天然药物中含有的酚酸。它们具有清除自由基和抑制血栓形成的生物活性。酚酸可以抑制低密度脂蛋白的氧化，还具有抗炎作用。本文首次报道了一种毛细管电泳 - 化学发光（CE - CL）方法，用于同时测定中药（包括银翘解毒片、双黄连口服液和颗粒剂）中发现的四种酚酸。在自行组装的CE - CL装置上进行毛细管电泳分离，使用未涂层熔融石英毛细管（有效长度66 cm，内径50 μm），背景电解质由3.0×10 M Ag(iii)（pH = 12.01）、3.0 mM鲁米诺（pH = 9.20）和10 mM硼酸钠溶液组成。进样时间为12 s（重力进样），分离电压为22 kV。固相萃取（SPE）和CE - CL的结合提高了灵敏度。在最佳条件下，绿原酸、阿魏酸、香草酸和咖啡酸的校准曲线线性范围分别为0.625至20.0、1.000至30.0、0.150至1.50和0.045至1.00 μg/mL。检测限为0.014至0.300 μg/mL。还验证了所提出的方法用于测定中药中四种目标分析物的实用性，回收率范围为90.9%至119.8%。综上所述，这些结果表明所开发的方法灵敏且可靠。此外，该方法成功应用于实际中药样品。
