Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan 430065, China.
School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, China.
J Pharm Biomed Anal. 2019 Oct 25;175:112734. doi: 10.1016/j.jpba.2019.06.031. Epub 2019 Jul 5.
A rapid and reliable LC-MS/MS method was developed for the quantitation of major components in Folium Artemisiae Argyi (mugwort), a widely used traditional Chinese herbal medicine. A total of 5 phenolic acids and 17 flavonoids were separated and simultaneously determined by using a Shiseido C column (150 × 3.0 mm, 3 μm) and gradient elution of acetonitrile-aqueous formic acid (100:0.1, v/v) at a 0.5 mL min flow rate, via multiple reaction monitor (MRM) in polarity switching mode. The quantitative method was validated in terms of sensitivity, linearity, precision, accuracy and stability, which proved to be sensitive, accurate and reproducible. Then 65 samples collected from different areas were selected for component analysis by LC-MS/MS and assessment of antioxidant activity using DPPH, ABTS, FRAP, O and OH scavenging assays. Grey relational analysis and partial least square regression were used to evaluate the relevance between chemicals and bioactivities, and the results indicated chlorogenic acid, isochlorogenic acid B, A, C, eriodictyol, jaceosidin and eupatilin made the key contribution to antioxidant activity. The present study combines chemical analysis and bioassay to identify bioactive markers, which possesses potential value for the activity-oriented quality control of mugwort.
建立了一种快速可靠的 LC-MS/MS 方法，用于定量分析艾蒿叶（艾草）中的主要成分，艾草是一种广泛使用的传统中药。采用 Shiseido C 柱（150×3.0mm，3μm）和乙腈-水甲酸（100：0.1，v/v）梯度洗脱，以 0.5 mL/min 的流速，通过多反应监测（MRM）在极性切换模式下，分离并同时测定 5 种酚酸和 17 种黄酮类化合物。从灵敏度、线性、精密度、准确度和稳定性等方面对定量方法进行了验证，结果表明该方法灵敏、准确、重现性好。然后，采用 LC-MS/MS 对 65 个来自不同地区的样品进行成分分析，并采用 DPPH、ABTS、FRAP、O 和 OH 清除测定法评估抗氧化活性。采用灰色关联分析和偏最小二乘回归法评价化学成分与生物活性之间的相关性，结果表明绿原酸、异绿原酸 B、A、C、圣草酚、芹菜素和木犀草素对抗氧化活性有重要贡献。本研究将化学分析与生物测定相结合，鉴定生物活性标志物，为艾蒿的活性导向质量控制提供了潜在价值。
