采用固相萃取法通过液相色谱法测定血清中环孢素A。临床研究中高效液相色谱法与放射免疫分析法水平的比较。

Liquid chromatographic determination of cyclosporin A in serum with use of a solid-phase extraction. Comparison between high-performance liquid chromatography and radioimmunoassay levels in clinical investigations.

作者信息

Brossat B, Straczek J, Heulin M H, Herbeuval X, Belleville F, Nabet P, Lokiec F

出版信息

J Chromatogr. 1987 Jan 23;413:141-50. doi: 10.1016/0378-4347(87)80221-9.

Abstract

A simple reliable liquid chromatographic method for assay of cyclosporin A in serum or urine is described. Samples were cleaned up on a solid-phase extraction system (cyanopropyl column). The system involved a reversed-phase C18 Ultrasphere column maintained at 72 degrees C and an acetonitrile linear gradient (65 to 95%) in 0.14% triethylammonium phosphate. Liquid chromatographic analysis of radioimmunoassay standards shows that some samples contain a contaminant peak. Comparison of cyclosporin A levels obtained by radioimmunoassay and high-performance liquid chromatography in clinical investigations show that the former values are generally, but not always, higher than the latter, and that cyclosporin A is very differently metabolized depending on the patient, disease and treatment.

摘要

本文描述了一种用于测定血清或尿液中环孢素A的简单可靠的液相色谱方法。样品在固相萃取系统（氰基丙基柱）上进行净化处理。该系统采用反相C18超球体柱，柱温保持在72℃，流动相为乙腈线性梯度（65%至95%），含0.14%的磷酸三乙铵。对放射免疫分析标准品进行液相色谱分析表明，一些样品含有杂质峰。在临床研究中，通过放射免疫分析和高效液相色谱法获得的环孢素A水平比较显示，前者的值通常（但并非总是）高于后者，并且环孢素A的代谢因患者、疾病和治疗方式的不同而有很大差异。