Nourbakhsh Fahimeh, Mousavi Seyed Hadi, Rahmanian-Devin Pouria, Baradaran Rahimi Vafa, Rakhshandeh Hassan, Askari Vahid Reza
Medical Toxicology Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Evid Based Complement Alternat Med. 2022 Apr 22;2022:3707647. doi: 10.1155/2022/3707647. eCollection 2022.
Psoriasis is considered an autoimmune inflammatory disease. The disease is spread and diagnosed by the infiltration of inflammatory mediators and cells into the epidermis. Recent theoretical developments have focused on the effectiveness of noscapine (NOS) as a potential alkaloid for being used as a valuable treatment for different diseases. In the present study, psoriasis-like dermatitis was induced on the right ear pinna surface of male mice by topical application of imiquimod (IMQ) for ten consecutive days, which was treated with noscapine (0.3, 1, 3, and 10% w/v) or clobetasol (0.05% w/v) as a positive control. The levels of ear length, thickness, severity of skin inflammation, psoriatic itch, psoriasis area severity index (PASI) score, and body weight were measured daily. On the 10 day of study, each ear was investigated for inflammation, fibrosis, proliferation, and apoptosis using histopathological (H&E and Masson's trichrome staining) and immunohistochemistry (Ki67 and p53 staining) assays. Furthermore, the levels of inflammatory biomarkers were characterized by an enzyme-linked immunosorbent assay (ELISA). The results confirmed IMQ-induced psoriasis for five consecutive days. In contrast, noscapine significantly reduced the ear length, thickness, severity of skin inflammation, psoriatic itch and body weight, tumor necrosis factor- (TNF-), transforming growth factor- (TGF-), interferon-gamma (IFN-), interleukin 6 (IL-6), IL-17, and IL-23p19 in a concentration-dependent manner ( < 0.001-0.05 for all cases). Overall, topical noscapine significantly ameliorated both the macroscopical and microscopical features of psoriasis. However, further clinical investigations are required to translate the effects to clinics.
银屑病被认为是一种自身免疫性炎症性疾病。该疾病通过炎症介质和细胞浸润到表皮而传播和诊断。最近的理论进展集中在那可丁（NOS）作为一种潜在生物碱在用于治疗不同疾病方面的有效性。在本研究中，通过连续10天局部应用咪喹莫特（IMQ）在雄性小鼠的右耳廓表面诱导出银屑病样皮炎，用那可丁（0.3、1、3和10% w/v）或作为阳性对照的氯倍他索（0.05% w/v）进行治疗。每天测量耳长、厚度、皮肤炎症严重程度、银屑病瘙痒、银屑病面积严重程度指数（PASI）评分和体重。在研究的第10天，使用组织病理学（苏木精和伊红染色以及Masson三色染色）和免疫组织化学（Ki67和p53染色）分析对每只耳朵进行炎症、纤维化、增殖和凋亡的研究。此外，通过酶联免疫吸附测定（ELISA）对炎症生物标志物的水平进行表征。结果证实连续5天IMQ诱导出银屑病。相比之下，那可丁以浓度依赖性方式显著降低了耳长、厚度、皮肤炎症严重程度、银屑病瘙痒和体重、肿瘤坏死因子-（TNF-）、转化生长因子-（TGF-）、干扰素-γ（IFN-）、白细胞介素6（IL-6）、IL-17和IL-23p19（所有情况均为<0.001 - 0.05）。总体而言，局部应用那可丁显著改善了银屑病的宏观和微观特征。然而，需要进一步的临床研究将这些效果转化到临床应用中。
