Yang Baiqi, Han Yanchao, Wu Weijie, Fang Xiangjun, Chen Hangjun, Gao Haiyan
Food Science Institute, Zhejiang Academy of Agricultural Sciences, Key Laboratory of Post-Harvest Handing of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Hangzhou 310021, China.
Food Chem X. 2022 Feb 12;13:100254. doi: 10.1016/j.fochx.2022.100254. eCollection 2022 Mar 30.
Melatonin, a crucial bioactive molecule, involved in several physiological processes in plants. This study investigated the effects of melatonin (MT) treatment on lignification, including firmness, lignin, lignified-enzyme activities, the expression patterns of genes encoding corresponding enzymes and transcription factors in water bamboo shoot during storage for 8 days. MT treatment decreased the firmness and content of lignin. It inhibited the degradation of total phenols and ascorbic acid and delayed the lignin biosynthesis, via reducing the activities of phenylalanine ammonia-lyse cinnamyl alcohol dehydrogenase and peroxidase, as well as lignin biosynthesis-related genes expression levels. Transcription factors of and from NAC family and and from MYB family were increased in water bamboo shoot after harvest and MT-treated markedly reduced their expression. Therefore, our findings supply a fundamental understanding of MT treatment suppression of lignification and establish a foundation for further research on transcriptional regulation.
褪黑素是一种关键的生物活性分子，参与植物的多种生理过程。本研究调查了褪黑素（MT）处理对茭白贮藏8天期间木质化的影响，包括硬度、木质素、木质化酶活性、编码相应酶和转录因子的基因表达模式。MT处理降低了硬度和木质素含量。它通过降低苯丙氨酸解氨酶、肉桂醇脱氢酶和过氧化物酶的活性以及木质素生物合成相关基因的表达水平，抑制了总酚和抗坏血酸的降解，并延迟了木质素生物合成。收获后茭白中NAC家族的 和 以及MYB家族的 和 的转录因子增加，而MT处理显著降低了它们的表达。因此，我们的研究结果为MT处理抑制木质化提供了基本认识，并为进一步研究转录调控奠定了基础。
