抗体嵌合体技术应用于大肠杆菌热不稳定肠毒素的检测。

Antibody chimera technique applied to the detection of Escherichia coli heat-labile enterotoxin.

作者信息

Germani Y, Guesdon J L, Bégaud E, Moreau J P

出版信息

J Immunol Methods. 1987 Apr 2;98(1):83-9. doi: 10.1016/0022-1759(87)90439-x.

Abstract

Covalently prepared chimera antibodies were tested in a ganglioside GM1 erythro-immunoassay (CERIA) for E. coli heat-labile enterotoxin (LT) detection. The antibody specific for LT was conjugated with a polyclonal antibody specific for sheep erythrocytes. The assay is based on the specific binding of LT to polystyrene-adsorbed GM1 and subsequent erythro-adsorption via chimera antibody by which the bound toxin is visualized. Enterotoxin titers determined with this CERIA method were similar to those obtained with the Vero cell assay and with ELISA. 5 ng of cholera toxin/ml may be detected with the assay. The CERIA, as described, may be used either qualitatively or quantitatively and is well suited for routine laboratory diagnosis of LT in a culture supernatant of E. coli.

摘要

共价制备的嵌合抗体在神经节苷脂GM1红细胞免疫测定法（CERIA）中进行了测试，用于检测大肠杆菌热不稳定肠毒素（LT）。针对LT的特异性抗体与针对绵羊红细胞的多克隆抗体偶联。该测定基于LT与聚苯乙烯吸附的GM1的特异性结合，以及随后通过嵌合抗体进行的红细胞吸附，通过这种方式可以观察到结合的毒素。用这种CERIA方法测定的肠毒素滴度与用Vero细胞测定法和ELISA获得的滴度相似。该测定法可检测出5 ng/ml的霍乱毒素。所述的CERIA可用于定性或定量检测，非常适合于大肠杆菌培养上清液中LT的常规实验室诊断。