Germani Y, Begaud E, Guesdon J L
Med Microbiol Immunol. 1987;176(2):89-97. doi: 10.1007/BF00200680.
We investigated the possibility of using a sheep erythrocyte-antibody conjugate as reagent in a sandwich erythroimmunoassay (SERIA) procedure to detect and titrate Escherichia coli heat-labile enterotoxin (LT) with the naked eye. In this assay, which is based on the immunological similarity between Vibrio cholerae toxin (CT) and LT, rabbit anti-CT IgG was used as immunosorbent, and sheep erythrocytes, sensitized with the rabbit anti-CT antibodies, were used as indicator. The sensitivity of the test was demonstrated by a comparative study using an enzyme-linked immunosorbent assay (ELISA). The results obtained by SERIA with 130 samples correlated well with those of a Vero cell assay and a GM1-ELISA. The test is easy, relatively cheap and as sensitive as other standard techniques; it is particularly suited for field laboratories, especially in tropical countries, and a large number of strains may be examined daily.
我们研究了在夹心红细胞免疫测定法（SERIA）中使用绵羊红细胞 - 抗体结合物作为试剂，以肉眼检测和滴定大肠杆菌不耐热肠毒素（LT）的可能性。在该测定法中，基于霍乱弧菌毒素（CT）和LT之间的免疫相似性，兔抗CT IgG用作免疫吸附剂，用兔抗CT抗体致敏的绵羊红细胞用作指示剂。通过使用酶联免疫吸附测定法（ELISA）的比较研究证明了该测试的灵敏度。用SERIA对130个样品获得的结果与Vero细胞测定法和GM1 - ELISA的结果相关性良好。该测试简便、相对便宜且与其他标准技术一样灵敏；它特别适合现场实验室，尤其是在热带国家，并且每天可以检测大量菌株。
