Personal Care, DSM Nutritional Products, Kaiseraugst, Switzerland.
Nutrition Innovation Center, DSM Nutritional Products, Lexington, Massachusetts, USA.
Int J Cosmet Sci. 2022 Aug;44(4):440-452. doi: 10.1111/ics.12777. Epub 2022 Jun 23.
Facial skin is a particularly complex environment made of different skin types such as sebaceous (forehead) and dry (cheeks). The skin microbiota composition on different facial sites has not yet been addressed.
We conducted a 4-week-long, single-centre, randomized and placebo-controlled clinical study involving 23 Caucasian females. We assessed both bacterial composition on five different facial areas and the microbiome modulatory effects resulting from the topical application of a plant extract (Epilobium fleischeri). Skin microbiome samples were collected before and after 4 weeks of product application. Microbiota profiling was performed via 16S rRNA gene sequencing, and relative abundance data were used to calculate differentials via a multinomial regression model.
Via 'reference frames', we observed shifts in microbial composition after 4 weeks of twice-daily product application and identify certain microbiota species, which were positively associated with the application of the product containing the Epilobium fleischeri extract. Staphylococcus hominis, Staphylococcus epidermidis, and Micrococcus yunnanensis appeared to be significantly enriched in the final microbiota composition of the active treatment group.
Facial skin was found to be colonized by an heterogenous microbiota, and the Epilobium fleischeri extract had a modulatory effect on commensal bacteria on the different facial sites.
面部皮肤是一个特别复杂的环境，由不同的皮肤类型组成，如油性（额头）和干性（脸颊）。不同面部部位的皮肤微生物群落组成尚未得到解决。
我们进行了一项为期 4 周的、单中心、随机和安慰剂对照的临床研究，涉及 23 名白种女性。我们评估了五个不同面部区域的细菌组成，以及植物提取物（柳叶菜）对面部微生物组的调节作用。在产品应用 4 周前后采集皮肤微生物组样本。通过 16S rRNA 基因测序进行微生物组分析，相对丰度数据通过多项回归模型计算差异。
通过“参考框架”，我们观察到产品应用 4 周后微生物组成发生了变化，并确定了某些与含有柳叶菜提取物的产品应用相关的微生物群落。金黄色葡萄球菌、表皮葡萄球菌和云南微球菌似乎在活性治疗组的最终微生物群落组成中明显富集。
面部皮肤被异质微生物定植，柳叶菜提取物对面部不同部位的共生菌具有调节作用。
