Takeya M, Hsiao L, Takahashi K
J Leukoc Biol. 1987 Mar;41(3):187-95. doi: 10.1002/jlb.41.3.187.
An anti-macrophage monoclonal antibody designated TRPM-3 was produced using thioglycollate-elicited rat peritoneal macrophages as immunogen. By the immunoperoxidase method, TRPM-3 was found to be specific for certain macrophage populations, such as marginal zone macrophages and marginal metallophils of the spleen, sinus macrophages of the lymph nodes, and omentum macrophages. No epithelial cells, peripheral blood monocytes, granulocytes, or lymphocytes had reacted with TRPM-3. Immunoelectron microscopically, reaction to TRPM-3 was clearly demonstrated on the plasma membrane of splenic marginal zone macrophages, lymphatic sinus macrophages, omentum macrophages, and peritoneal macrophages. Accordingly, TRPM-3 was considered to have recognized the particular membrane antigen expressed by restricted macrophage populations.
以巯基乙酸盐诱导的大鼠腹腔巨噬细胞作为免疫原，制备了一种名为TRPM - 3的抗巨噬细胞单克隆抗体。通过免疫过氧化物酶法发现，TRPM - 3对某些巨噬细胞群体具有特异性，如脾脏的边缘区巨噬细胞和边缘金属嗜细胞、淋巴结的窦巨噬细胞以及网膜巨噬细胞。上皮细胞、外周血单核细胞、粒细胞或淋巴细胞均未与TRPM - 3发生反应。免疫电子显微镜观察显示，脾边缘区巨噬细胞、淋巴窦巨噬细胞、网膜巨噬细胞和腹腔巨噬细胞的质膜上均有明显的TRPM - 3反应。因此，TRPM - 3被认为识别了特定巨噬细胞群体所表达的特殊膜抗原。
