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生物发光增强酶免疫测定法。酶免疫测定的新型超灵敏检测系统,II。

Bioluminescence enhanced enzyme immunoassay. New ultrasensitive detection systems for enzyme immunoassays, II.

作者信息

Geiger R, Miska W

出版信息

J Clin Chem Clin Biochem. 1987 Jan;25(1):31-8. doi: 10.1515/cclm.1987.25.1.31.

DOI:10.1515/cclm.1987.25.1.31
PMID:3549963
Abstract

Ultrasensitive bioluminescence immunoassays for the determination of peptides and proteins (illustrated with human urinary kallikrein, bradykinin and the determination of human urinary kallikrein antibody titers) have been developed. The usable ranges of the standard curves are from 5 pg to 5000 pg per liter. The relative intra-assay coefficients of variation of the tests were between 2 and 6%, and the interassay coefficients of variation between 4 and 12%.

摘要

已开发出用于测定肽和蛋白质的超灵敏生物发光免疫测定法(以人尿激肽释放酶、缓激肽为例,并用于测定人尿激肽释放酶抗体滴度)。标准曲线的可用范围为每升5皮克至5000皮克。试验的相对批内变异系数在2%至6%之间,批间变异系数在4%至12%之间。

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Bioluminescence enhanced enzyme immunoassay. New ultrasensitive detection systems for enzyme immunoassays, II.生物发光增强酶免疫测定法。酶免疫测定的新型超灵敏检测系统,II。
J Clin Chem Clin Biochem. 1987 Jan;25(1):31-8. doi: 10.1515/cclm.1987.25.1.31.
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