Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, Brazil.
Department of Animal Sciences, Universidade Federal Rural do Semi-Árido - UFERSA, Mossoró, Brazil.
Reprod Domest Anim. 2022 Aug;57(8):912-918. doi: 10.1111/rda.14140. Epub 2022 May 11.
We studied the sperm membrane functionality through the epididymal transit by comparing different hypoosmotic solutions and verifying possible associations among osmotic response and functional parameters of sperm in red-rumped agouti (Dasyprocta leporina). For this purpose, epididymal sperm from six sexually mature male agoutis were collected via flotation. Then, analyses of sperm parameters and hypoosmotic swelling test using different hypoosmotic solutions (0, 50 and 200 mOsm/L) in different regions of the epididymis (caput, corpus and cauda) were performed. There was an increase (p < .05) in the values for sperm concentration, the total number of sperm recovered, total and progressive motility, average path velocity, straight-line velocity, curvilinear velocity, and rapid and medium subpopulations following the caput-corpus-cauda direction. Regardless of the hypoosmotic solution, the agouti sperm membrane presented similar functional integrity in all the epididymal regions. Moreover, the highest (p < .05) osmotic responses were reached with the use of 50 mOsm/L solution in comparison to 0 and 200 mOsm/L for all the regions. Significant correlations among osmotic response and some sperm kinetic parameters were observed, especially in epididymal caput, while no correlations were found in the region of the cauda. In summary, red-rumped agouti sperm present similar membrane functionality during epididymal transit, but there are evident correlations among such functionality and sperm kinetic parameters, especially in the caput region. Moreover, we indicate the use of a 50 mOsm/L hypoosmotic solution for the analysis of this parameter through the hypoosmotic swelling test.
我们通过比较不同的低渗溶液，研究了精子膜功能通过附睾转运，验证了红臀豚鼠（Dasyprocta leporina）精子渗透反应与功能参数之间的可能关联。为此，通过漂浮法从 6 只性成熟雄性红臀豚鼠中收集附睾精子。然后，对不同低渗溶液（0、50 和 200 mOsm/L）在附睾头、体和尾不同区域的精子参数和低渗肿胀试验进行分析。精子浓度、总精子回收数、总活力、前向运动活力、平均路径速度、直线速度、曲线速度、快速和中等亚群均随着附睾头-体-尾的方向增加（p <.05）。无论低渗溶液如何，豚鼠精子膜在所有附睾区域均表现出相似的功能完整性。此外，与 0 和 200 mOsm/L 相比，在所有区域中，使用 50 mOsm/L 溶液时渗透反应最高（p <.05）。在附睾头观察到渗透反应与一些精子动力学参数之间存在显著相关性，而在尾部区域则没有相关性。总之，红臀豚鼠精子在附睾转运过程中具有相似的膜功能，但在功能和精子动力学参数之间存在明显的相关性，尤其是在附睾头区域。此外，我们建议使用 50 mOsm/L 的低渗溶液通过低渗肿胀试验来分析该参数。
