采用 p-氨基苯硫酚重氮化偶联后，利用负载 Ag/Au 纳米贵金属的金属有机框架对多巴胺进行 SERS 测定。
SERS determination of dopamine using metal-organic frameworks decorated with Ag/Au noble metal nanoparticle composite after azo derivatization with p-aminothiophenol.
机构信息
The Education Ministry Key Lab of Resource Chemistry, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China.
出版信息
Mikrochim Acta. 2022 May 2;189(5):207. doi: 10.1007/s00604-022-05292-8.
A specific surface-enhanced Raman scattering (SERS) assay for dopamine (DA) based on an azo derivatization reaction is proposed for the first time by preparation of p-aminothiophenol (PATP)-modified composite SERS substrate, composed of metal-organic framework (MIL-101) decorated with Au and Ag nanoparticles. As the result, the SERS method for detection of the azo reaction between PATP and DA exhibits superior sensitivity, selectivity, and stability. A reasonable linearity in the range 10 to 10 mol∙L is achieved, and the limit of detection is 1.2 × 10 mol∙L. The reactive SERS assay is free from interference in complex physiological fluid. The feasibility of the proposed SERS method for the detection of DA levels in fetal bovine serum (FBS) samples and human serum samples is validated by HPLC-MS methods, displaying promising application potential in early disease diagnosis.
首次提出了一种基于偶联反应的特定表面增强拉曼散射（SERS）测定多巴胺（DA）的方法，通过制备由金属有机骨架（MIL-101）修饰的金和银纳米粒子的 p-氨基硫酚（PATP）修饰的复合 SERS 基底来实现。结果表明，用于检测 PATP 和 DA 之间偶联反应的 SERS 方法具有优异的灵敏度、选择性和稳定性。在 10 到 10 mol·L 的范围内实现了合理的线性关系，检测限为 1.2×10 mol·L。该反应性 SERS 测定法不受复杂生理流体的干扰。通过高效液相色谱-质谱（HPLC-MS）方法验证了所提出的 SERS 方法检测胎牛血清（FBS）样品和人血清样品中 DA 水平的可行性，显示出在早期疾病诊断中的应用潜力。
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