Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia.
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
Bull Exp Biol Med. 2022 Apr;172(6):691-694. doi: 10.1007/s10517-022-05458-7. Epub 2022 May 2.
Experimental type 1 diabetes mellitus (T1DM) was induced in rats by daily intraperitoneal injections of alloxan in a dose of 90 mg/kg for 4 days. For verification of insulin resistance, insulin tolerance test was performed in 2 weeks and the glucose utilization rate constant (K) was calculated. The rats demonstrated the main symptoms of T1DM: hypoinsulinemia, hyperglycemia, ketonemia, glucosuria, ketonuria, polydipsia, polyphagia, weight loss, and insulin resistance, as evidenced by a decrease in K. The serum content of free fatty acids and triacylglycerols significantly increased. The content of triacylglycerols increased in skeletal muscles and decreased in the liver. A negative linear correlation was found between K and triacylglycerol content in muscles. Thus, the development of insulin resistance in experimental T1DM in rats is associated with accumulation of triacylglycerols in skeletal muscles.
实验性 1 型糖尿病（T1DM）通过每天腹腔注射 90mg/kg 的链脲佐菌素连续 4 天诱导大鼠产生。为了验证胰岛素抵抗，在 2 周时进行胰岛素耐量试验，并计算葡萄糖利用率常数（K）。大鼠表现出 T1DM 的主要症状：低胰岛素血症、高血糖、酮血症、糖尿、酮尿、多饮、多食、体重减轻和胰岛素抵抗，表现为 K 值降低。血清游离脂肪酸和三酰甘油含量显著增加。骨骼肌中的三酰甘油含量增加，而肝脏中的三酰甘油含量减少。K 值与肌肉中三酰甘油含量呈负线性相关。因此，大鼠实验性 T1DM 中胰岛素抵抗的发展与骨骼肌中三酰甘油的积累有关。
