Xu Chengran, Huang Jinhai, Yang Yi, Li Lun, Li Guangyu
Department of Neurosurgery, First Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
Department of Neurosurgery, Anshan Hospital of the First Hospital of China Medical University, Anshan, People's Republic of China.
Int J Gen Med. 2022 Apr 26;15:4399-4407. doi: 10.2147/IJGM.S350454. eCollection 2022.
The homeobox gene 5 () encodes a transcription factor that regulates the embryonic development of the central nervous system. Notably, its expression pattern and prognostic role in glioma remain unelucidated.
This study identified the relationship between HOXB5 and glioma by investigating expression data from The Cancer Genome Atlas and the Genotype Tissue Expression databases and validating the obtained data using the Chinese Glioma Genome Atlas database. Western blots were used to identify HOXB5 expression levels in glioma cells and clinical samples. Kaplan-Meier and multivariate Cox regression analyses were performed to assess the prognostic value of HOXB5. The key functions and signaling pathways related to HOXB5 were analyzed using GO, KEGG, and GSEA. Immune infiltration was calculated using the microenvironment cell populations-counter, estimate the proportion of immune and cancer, and ESTIMATE algorithms.
The expression of HOXB5 was upregulated in glioma and generally increased with malignancy. HOXB5 was an independent prognostic factor for glioma patients. A nomogram was further built that integrated HOXB5, and it showed stratifying prediction accuracy and efficiency. HOXB5 was associated with the regulation of cell growth, endothelial cell growth, and the IL-6/JAK-STAT3 pathway, and was determined to possibly promote stomatal specimen enrichment and angiogenesis.
HOXB5 protein is overexpressed in glioma and might serve as a good predictive factor of this disease.
同源盒基因5（HOXB5）编码一种调节中枢神经系统胚胎发育的转录因子。值得注意的是，其在胶质瘤中的表达模式和预后作用仍未阐明。
本研究通过调查癌症基因组图谱和基因型组织表达数据库中的HOXB5表达数据，并使用中国胶质瘤基因组图谱数据库验证所获数据，确定HOXB5与胶质瘤之间的关系。采用蛋白质免疫印迹法确定胶质瘤细胞和临床样本中HOXB5的表达水平。进行Kaplan-Meier和多变量Cox回归分析以评估HOXB5的预后价值。使用基因本体论（GO）、京都基因与基因组百科全书（KEGG）和基因集富集分析（GSEA）分析与HOXB5相关的关键功能和信号通路。使用微环境细胞群体计数器、免疫细胞与癌细胞比例估算以及ESTIMATE算法计算免疫浸润情况。
HOXB5在胶质瘤中的表达上调，且通常随恶性程度增加而升高。HOXB5是胶质瘤患者的独立预后因素。进一步构建了整合HOXB5的列线图，其显示出分层预测的准确性和效率。HOXB5与细胞生长、内皮细胞生长以及白细胞介素-6/信号转导子和转录激活子3（IL-6/JAK-STAT3）通路的调节相关，并被确定可能促进气孔标本富集和血管生成。
HOXB5蛋白在胶质瘤中过表达，可能是该疾病的良好预测因子。
